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Amniocytes can serve a dual function as a source of iPS cells and feeder layers
Clinical barriers to stem-cell therapy include the need for efficient derivation of histocompatible stem cells and the zoonotic risk inherent to human stem-cell xenoculture on mouse feeder cells. We describe a system for efficiently deriving induced pluripotent stem (iPS) cells from human and mouse...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033187/ https://www.ncbi.nlm.nih.gov/pubmed/21156717 http://dx.doi.org/10.1093/hmg/ddq542 |
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author | Anchan, Raymond M. Quaas, Philipp Gerami-Naini, Behzad Bartake, Hrishikesh Griffin, Adam Zhou, Yilan Day, Daniel Eaton, Jennifer L. George, Liji L. Naber, Catherine Turbe-Doan, Annick Park, Peter J. Hornstein, Mark D. Maas, Richard L. |
author_facet | Anchan, Raymond M. Quaas, Philipp Gerami-Naini, Behzad Bartake, Hrishikesh Griffin, Adam Zhou, Yilan Day, Daniel Eaton, Jennifer L. George, Liji L. Naber, Catherine Turbe-Doan, Annick Park, Peter J. Hornstein, Mark D. Maas, Richard L. |
author_sort | Anchan, Raymond M. |
collection | PubMed |
description | Clinical barriers to stem-cell therapy include the need for efficient derivation of histocompatible stem cells and the zoonotic risk inherent to human stem-cell xenoculture on mouse feeder cells. We describe a system for efficiently deriving induced pluripotent stem (iPS) cells from human and mouse amniocytes, and for maintaining the pluripotency of these iPS cells on mitotically inactivated feeder layers prepared from the same amniocytes. Both cellular components of this system are thus autologous to a single donor. Moreover, the use of human feeder cells reduces the risk of zoonosis. Generation of iPS cells using retroviral vectors from short- or long-term cultured human and mouse amniocytes using four factors, or two factors in mouse, occurs in 5–7 days with 0.5% efficiency. This efficiency is greater than that reported for mouse and human fibroblasts using similar viral infection approaches, and does not appear to result from selective reprogramming of Oct4(+) or c-Kit(+) amniocyte subpopulations. Derivation of amniocyte-derived iPS (AdiPS) cell colonies, which express pluripotency markers and exhibit appropriate microarray expression and DNA methylation properties, was facilitated by live immunostaining. AdiPS cells also generate embryoid bodies in vitro and teratomas in vivo. Furthermore, mouse and human amniocytes can serve as feeder layers for iPS cells and for mouse and human embryonic stem (ES) cells. Thus, human amniocytes provide an efficient source of autologous iPS cells and, as feeder cells, can also maintain iPS and ES cell pluripotency without the safety concerns associated with xenoculture. |
format | Text |
id | pubmed-3033187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30331872011-02-03 Amniocytes can serve a dual function as a source of iPS cells and feeder layers Anchan, Raymond M. Quaas, Philipp Gerami-Naini, Behzad Bartake, Hrishikesh Griffin, Adam Zhou, Yilan Day, Daniel Eaton, Jennifer L. George, Liji L. Naber, Catherine Turbe-Doan, Annick Park, Peter J. Hornstein, Mark D. Maas, Richard L. Hum Mol Genet Articles Clinical barriers to stem-cell therapy include the need for efficient derivation of histocompatible stem cells and the zoonotic risk inherent to human stem-cell xenoculture on mouse feeder cells. We describe a system for efficiently deriving induced pluripotent stem (iPS) cells from human and mouse amniocytes, and for maintaining the pluripotency of these iPS cells on mitotically inactivated feeder layers prepared from the same amniocytes. Both cellular components of this system are thus autologous to a single donor. Moreover, the use of human feeder cells reduces the risk of zoonosis. Generation of iPS cells using retroviral vectors from short- or long-term cultured human and mouse amniocytes using four factors, or two factors in mouse, occurs in 5–7 days with 0.5% efficiency. This efficiency is greater than that reported for mouse and human fibroblasts using similar viral infection approaches, and does not appear to result from selective reprogramming of Oct4(+) or c-Kit(+) amniocyte subpopulations. Derivation of amniocyte-derived iPS (AdiPS) cell colonies, which express pluripotency markers and exhibit appropriate microarray expression and DNA methylation properties, was facilitated by live immunostaining. AdiPS cells also generate embryoid bodies in vitro and teratomas in vivo. Furthermore, mouse and human amniocytes can serve as feeder layers for iPS cells and for mouse and human embryonic stem (ES) cells. Thus, human amniocytes provide an efficient source of autologous iPS cells and, as feeder cells, can also maintain iPS and ES cell pluripotency without the safety concerns associated with xenoculture. Oxford University Press 2011-03-01 2010-12-14 /pmc/articles/PMC3033187/ /pubmed/21156717 http://dx.doi.org/10.1093/hmg/ddq542 Text en © The Author 2010. Published by Oxford University Press http://creativecommons.org/licenses/by-nc/2.5/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Anchan, Raymond M. Quaas, Philipp Gerami-Naini, Behzad Bartake, Hrishikesh Griffin, Adam Zhou, Yilan Day, Daniel Eaton, Jennifer L. George, Liji L. Naber, Catherine Turbe-Doan, Annick Park, Peter J. Hornstein, Mark D. Maas, Richard L. Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title | Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title_full | Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title_fullStr | Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title_full_unstemmed | Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title_short | Amniocytes can serve a dual function as a source of iPS cells and feeder layers |
title_sort | amniocytes can serve a dual function as a source of ips cells and feeder layers |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033187/ https://www.ncbi.nlm.nih.gov/pubmed/21156717 http://dx.doi.org/10.1093/hmg/ddq542 |
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