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Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains

BACKGROUND: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we used a label-free quantitative proteomic approach to estimate differences in p...

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Autores principales: Målen, Hiwa, De Souza, Gustavo A, Pathak, Sharad, Søfteland, Tina, Wiker, Harald G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033788/
https://www.ncbi.nlm.nih.gov/pubmed/21261938
http://dx.doi.org/10.1186/1471-2180-11-18
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author Målen, Hiwa
De Souza, Gustavo A
Pathak, Sharad
Søfteland, Tina
Wiker, Harald G
author_facet Målen, Hiwa
De Souza, Gustavo A
Pathak, Sharad
Søfteland, Tina
Wiker, Harald G
author_sort Målen, Hiwa
collection PubMed
description BACKGROUND: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we used a label-free quantitative proteomic approach to estimate differences in protein abundance between two closely related M. tuberculosis strains; the virulent H37Rv strain and its attenuated counterpart H37Ra. RESULTS: We were able to identify more than 1700 proteins from both strains. As expected, the majority of the identified proteins had similar relative abundance in the two strains. However, 29 membrane-associated proteins were observed with a 5 or more fold difference in their relative abundance in one strain compared to the other. Of note, 19 membrane- and lipo-proteins had higher abundance in H37Rv, while another 10 proteins had a higher abundance in H37Ra. Interestingly, the possible protein-export membrane protein SecF (Rv2586c), and three ABC-transporter proteins (Rv0933, Rv1273c and Rv1819c) were among the more abundant proteins in M. tuberculosis H37Rv. CONCLUSION: Our data suggests that the bacterial secretion system and the transmembrane transport system may be important determinants of the ability of distinct M. tuberculosis strains to cause disease.
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spelling pubmed-30337882011-02-05 Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains Målen, Hiwa De Souza, Gustavo A Pathak, Sharad Søfteland, Tina Wiker, Harald G BMC Microbiol Research Article BACKGROUND: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we used a label-free quantitative proteomic approach to estimate differences in protein abundance between two closely related M. tuberculosis strains; the virulent H37Rv strain and its attenuated counterpart H37Ra. RESULTS: We were able to identify more than 1700 proteins from both strains. As expected, the majority of the identified proteins had similar relative abundance in the two strains. However, 29 membrane-associated proteins were observed with a 5 or more fold difference in their relative abundance in one strain compared to the other. Of note, 19 membrane- and lipo-proteins had higher abundance in H37Rv, while another 10 proteins had a higher abundance in H37Ra. Interestingly, the possible protein-export membrane protein SecF (Rv2586c), and three ABC-transporter proteins (Rv0933, Rv1273c and Rv1819c) were among the more abundant proteins in M. tuberculosis H37Rv. CONCLUSION: Our data suggests that the bacterial secretion system and the transmembrane transport system may be important determinants of the ability of distinct M. tuberculosis strains to cause disease. BioMed Central 2011-01-24 /pmc/articles/PMC3033788/ /pubmed/21261938 http://dx.doi.org/10.1186/1471-2180-11-18 Text en Copyright ©2011 Målen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Målen, Hiwa
De Souza, Gustavo A
Pathak, Sharad
Søfteland, Tina
Wiker, Harald G
Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title_full Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title_fullStr Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title_full_unstemmed Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title_short Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
title_sort comparison of membrane proteins of mycobacterium tuberculosis h37rv and h37ra strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033788/
https://www.ncbi.nlm.nih.gov/pubmed/21261938
http://dx.doi.org/10.1186/1471-2180-11-18
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