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Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis
Sequencing of 16S rDNA, and of sodA(int) and rpoB(int) in some cases, was applied to DNA from heart valves of 46 patients (36 with definite and 10 with possible endocarditis). Sequence-based identifications were compared with those obtained with conventional methods. Among the 36 definite cases, 30...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Centers for Disease Control and Prevention
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034331/ https://www.ncbi.nlm.nih.gov/pubmed/14720393 http://dx.doi.org/10.3201/eid0912.030229 |
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author | Podglajen, Isabelle Bellery, Fabienne Poyart, Claire Coudol, Philippe Buu-Hoï, Annie Bruneval, Patrick Mainardi, Jean-Luc |
author_facet | Podglajen, Isabelle Bellery, Fabienne Poyart, Claire Coudol, Philippe Buu-Hoï, Annie Bruneval, Patrick Mainardi, Jean-Luc |
author_sort | Podglajen, Isabelle |
collection | PubMed |
description | Sequencing of 16S rDNA, and of sodA(int) and rpoB(int) in some cases, was applied to DNA from heart valves of 46 patients (36 with definite and 10 with possible endocarditis). Sequence-based identifications were compared with those obtained with conventional methods. Among the 36 definite cases, 30 had positive blood cultures and 6 had negative cultures. Among the 30 positive cases, sequencing of 16S rDNA permitted identification of species (18), genus (8), or neither (4); sodA(int) and rpoB(int) sequencing was necessary for species identification in 8 cases. Species identifications were identical in only 61.5%, when conventional techniques and DNA sequencing were used. In five of the six blood culture–negative endocarditis cases, sequencing identified Bartonella quintana (3), B. henselae (1), and Streptococcus gallolyticus (1). Our results demonstrate a clear benefit of molecular identification, particularly in cases of blood culture–negative endocarditis and of possible endocarditis, to confirm or invalidate the diagnosis. Moreover, in 19.4% of the definite cases, the improvement in species identification by sequencing led to improved patient management. |
format | Text |
id | pubmed-3034331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-30343312011-02-10 Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis Podglajen, Isabelle Bellery, Fabienne Poyart, Claire Coudol, Philippe Buu-Hoï, Annie Bruneval, Patrick Mainardi, Jean-Luc Emerg Infect Dis Research Sequencing of 16S rDNA, and of sodA(int) and rpoB(int) in some cases, was applied to DNA from heart valves of 46 patients (36 with definite and 10 with possible endocarditis). Sequence-based identifications were compared with those obtained with conventional methods. Among the 36 definite cases, 30 had positive blood cultures and 6 had negative cultures. Among the 30 positive cases, sequencing of 16S rDNA permitted identification of species (18), genus (8), or neither (4); sodA(int) and rpoB(int) sequencing was necessary for species identification in 8 cases. Species identifications were identical in only 61.5%, when conventional techniques and DNA sequencing were used. In five of the six blood culture–negative endocarditis cases, sequencing identified Bartonella quintana (3), B. henselae (1), and Streptococcus gallolyticus (1). Our results demonstrate a clear benefit of molecular identification, particularly in cases of blood culture–negative endocarditis and of possible endocarditis, to confirm or invalidate the diagnosis. Moreover, in 19.4% of the definite cases, the improvement in species identification by sequencing led to improved patient management. Centers for Disease Control and Prevention 2003-12 /pmc/articles/PMC3034331/ /pubmed/14720393 http://dx.doi.org/10.3201/eid0912.030229 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Podglajen, Isabelle Bellery, Fabienne Poyart, Claire Coudol, Philippe Buu-Hoï, Annie Bruneval, Patrick Mainardi, Jean-Luc Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title | Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title_full | Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title_fullStr | Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title_full_unstemmed | Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title_short | Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis |
title_sort | comparative molecular and microbiologic diagnosis of bacterial endocarditis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034331/ https://www.ncbi.nlm.nih.gov/pubmed/14720393 http://dx.doi.org/10.3201/eid0912.030229 |
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