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Use of a Western blot technique for the serodiagnosis of glanders
BACKGROUND: The in vivo diagnosis of glanders relies on the highly sensitive complement fixation test (CFT). Frequently observed false positive results are troublesome for veterinary authorities and cause financial losses to animal owners. Consequently, there is an urgent need to develop a test with...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034690/ https://www.ncbi.nlm.nih.gov/pubmed/21247488 http://dx.doi.org/10.1186/1746-6148-7-4 |
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author | Elschner, Mandy C Scholz, Holger C Melzer, Falk Saqib, Muhammad Marten, Peggy Rassbach, Astrid Dietzsch, Michael Schmoock, Gernot de Assis Santana, Vania L de Souza, Marcilia MA Wernery, Renate Wernery, Ulrich Neubauer, Heinrich |
author_facet | Elschner, Mandy C Scholz, Holger C Melzer, Falk Saqib, Muhammad Marten, Peggy Rassbach, Astrid Dietzsch, Michael Schmoock, Gernot de Assis Santana, Vania L de Souza, Marcilia MA Wernery, Renate Wernery, Ulrich Neubauer, Heinrich |
author_sort | Elschner, Mandy C |
collection | PubMed |
description | BACKGROUND: The in vivo diagnosis of glanders relies on the highly sensitive complement fixation test (CFT). Frequently observed false positive results are troublesome for veterinary authorities and cause financial losses to animal owners. Consequently, there is an urgent need to develop a test with high specificity. Hence, a Western blot assay making use of a partly purified lipopolysaccaride (LPS) containing antigen of three Burkholderia mallei strains was developed. The test was validated investigating a comprehensive set of positive and negative sera obtained from horses and mules from endemic and non endemic areas. RESULTS: The developed Western blot assay showed a markedly higher diagnostic specificity when compared to the prescribed CFT and therefore can be used as a confirmatory test. However, the CFT remains the test of choice for routine testing of glanders due to its high sensitivity, its feasibility using standard laboratory equipment and its worldwide distribution in diagnostic laboratories. CONCLUSIONS: The CFT should be amended by the newly validated Western blot to increase the positive likelihood ratio of glanders serodiagnosis in non endemic areas or areas with low glanders prevalence. Its use for international trade of horses and mules should be implemented by the OIE. |
format | Text |
id | pubmed-3034690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30346902011-02-08 Use of a Western blot technique for the serodiagnosis of glanders Elschner, Mandy C Scholz, Holger C Melzer, Falk Saqib, Muhammad Marten, Peggy Rassbach, Astrid Dietzsch, Michael Schmoock, Gernot de Assis Santana, Vania L de Souza, Marcilia MA Wernery, Renate Wernery, Ulrich Neubauer, Heinrich BMC Vet Res Methodology Article BACKGROUND: The in vivo diagnosis of glanders relies on the highly sensitive complement fixation test (CFT). Frequently observed false positive results are troublesome for veterinary authorities and cause financial losses to animal owners. Consequently, there is an urgent need to develop a test with high specificity. Hence, a Western blot assay making use of a partly purified lipopolysaccaride (LPS) containing antigen of three Burkholderia mallei strains was developed. The test was validated investigating a comprehensive set of positive and negative sera obtained from horses and mules from endemic and non endemic areas. RESULTS: The developed Western blot assay showed a markedly higher diagnostic specificity when compared to the prescribed CFT and therefore can be used as a confirmatory test. However, the CFT remains the test of choice for routine testing of glanders due to its high sensitivity, its feasibility using standard laboratory equipment and its worldwide distribution in diagnostic laboratories. CONCLUSIONS: The CFT should be amended by the newly validated Western blot to increase the positive likelihood ratio of glanders serodiagnosis in non endemic areas or areas with low glanders prevalence. Its use for international trade of horses and mules should be implemented by the OIE. BioMed Central 2011-01-19 /pmc/articles/PMC3034690/ /pubmed/21247488 http://dx.doi.org/10.1186/1746-6148-7-4 Text en Copyright ©2011 Elschner et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Elschner, Mandy C Scholz, Holger C Melzer, Falk Saqib, Muhammad Marten, Peggy Rassbach, Astrid Dietzsch, Michael Schmoock, Gernot de Assis Santana, Vania L de Souza, Marcilia MA Wernery, Renate Wernery, Ulrich Neubauer, Heinrich Use of a Western blot technique for the serodiagnosis of glanders |
title | Use of a Western blot technique for the serodiagnosis of glanders |
title_full | Use of a Western blot technique for the serodiagnosis of glanders |
title_fullStr | Use of a Western blot technique for the serodiagnosis of glanders |
title_full_unstemmed | Use of a Western blot technique for the serodiagnosis of glanders |
title_short | Use of a Western blot technique for the serodiagnosis of glanders |
title_sort | use of a western blot technique for the serodiagnosis of glanders |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034690/ https://www.ncbi.nlm.nih.gov/pubmed/21247488 http://dx.doi.org/10.1186/1746-6148-7-4 |
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