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A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034921/ https://www.ncbi.nlm.nih.gov/pubmed/21331172 http://dx.doi.org/10.1155/2011/982186 |
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author | Di, Youjun Zhao, Min Nie, Yingchun Wang, Fei Lv, Jiagen |
author_facet | Di, Youjun Zhao, Min Nie, Yingchun Wang, Fei Lv, Jiagen |
author_sort | Di, Youjun |
collection | PubMed |
description | A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K(3)Fe(CN)(6) reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time (t(R)) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10(−7) ~ 1.0 × 10(−4) mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples. |
format | Text |
id | pubmed-3034921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30349212011-02-17 A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement Di, Youjun Zhao, Min Nie, Yingchun Wang, Fei Lv, Jiagen J Autom Methods Manag Chem Research Article A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K(3)Fe(CN)(6) reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time (t(R)) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10(−7) ~ 1.0 × 10(−4) mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples. Hindawi Publishing Corporation 2011 2011-01-20 /pmc/articles/PMC3034921/ /pubmed/21331172 http://dx.doi.org/10.1155/2011/982186 Text en Copyright © 2011 Youjun Di et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Di, Youjun Zhao, Min Nie, Yingchun Wang, Fei Lv, Jiagen A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title | A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title_full | A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title_fullStr | A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title_full_unstemmed | A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title_short | A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement |
title_sort | high-performance liquid chromatography:chemiluminescence method for potential determination of vardenafil in dietary supplement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034921/ https://www.ncbi.nlm.nih.gov/pubmed/21331172 http://dx.doi.org/10.1155/2011/982186 |
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