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A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement

A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic...

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Detalles Bibliográficos
Autores principales: Di, Youjun, Zhao, Min, Nie, Yingchun, Wang, Fei, Lv, Jiagen
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034921/
https://www.ncbi.nlm.nih.gov/pubmed/21331172
http://dx.doi.org/10.1155/2011/982186
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author Di, Youjun
Zhao, Min
Nie, Yingchun
Wang, Fei
Lv, Jiagen
author_facet Di, Youjun
Zhao, Min
Nie, Yingchun
Wang, Fei
Lv, Jiagen
author_sort Di, Youjun
collection PubMed
description A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K(3)Fe(CN)(6) reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time (t(R)) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10(−7) ~ 1.0 × 10(−4) mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.
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spelling pubmed-30349212011-02-17 A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement Di, Youjun Zhao, Min Nie, Yingchun Wang, Fei Lv, Jiagen J Autom Methods Manag Chem Research Article A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K(3)Fe(CN)(6) reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time (t(R)) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10(−7) ~ 1.0 × 10(−4) mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples. Hindawi Publishing Corporation 2011 2011-01-20 /pmc/articles/PMC3034921/ /pubmed/21331172 http://dx.doi.org/10.1155/2011/982186 Text en Copyright © 2011 Youjun Di et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Di, Youjun
Zhao, Min
Nie, Yingchun
Wang, Fei
Lv, Jiagen
A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title_full A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title_fullStr A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title_full_unstemmed A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title_short A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
title_sort high-performance liquid chromatography:chemiluminescence method for potential determination of vardenafil in dietary supplement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034921/
https://www.ncbi.nlm.nih.gov/pubmed/21331172
http://dx.doi.org/10.1155/2011/982186
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