Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice

Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(−/−)Jak3(−/−)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood...

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Detalles Bibliográficos
Autores principales: Ono, Ayumi, Hattori, Shinichiro, Kariya, Ryusho, Iwanaga, Sumako, Taura, Manabu, Harada, Hideki, Suzu, Shinya, Okada, Seiji
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035318/
https://www.ncbi.nlm.nih.gov/pubmed/21331358
http://dx.doi.org/10.1155/2011/539748
Descripción
Sumario:Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(−/−)Jak3(−/−)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34(+) hematopoietic stem cells were successfully engrafted into Balb/c Rag-2(−/−)Jak3(−/−) mice; however, the engraftment rate was far lower in C57/BL6 Rag-2(−/−)Jak3(−/−) mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment.

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