Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice

Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(−/−)Jak3(−/−)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood...

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Autores principales: Ono, Ayumi, Hattori, Shinichiro, Kariya, Ryusho, Iwanaga, Sumako, Taura, Manabu, Harada, Hideki, Suzu, Shinya, Okada, Seiji
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035318/
https://www.ncbi.nlm.nih.gov/pubmed/21331358
http://dx.doi.org/10.1155/2011/539748
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author Ono, Ayumi
Hattori, Shinichiro
Kariya, Ryusho
Iwanaga, Sumako
Taura, Manabu
Harada, Hideki
Suzu, Shinya
Okada, Seiji
author_facet Ono, Ayumi
Hattori, Shinichiro
Kariya, Ryusho
Iwanaga, Sumako
Taura, Manabu
Harada, Hideki
Suzu, Shinya
Okada, Seiji
author_sort Ono, Ayumi
collection PubMed
description Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(−/−)Jak3(−/−)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34(+) hematopoietic stem cells were successfully engrafted into Balb/c Rag-2(−/−)Jak3(−/−) mice; however, the engraftment rate was far lower in C57/BL6 Rag-2(−/−)Jak3(−/−) mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment.
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spelling pubmed-30353182011-02-17 Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice Ono, Ayumi Hattori, Shinichiro Kariya, Ryusho Iwanaga, Sumako Taura, Manabu Harada, Hideki Suzu, Shinya Okada, Seiji J Biomed Biotechnol Research Article Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(−/−)Jak3(−/−)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34(+) hematopoietic stem cells were successfully engrafted into Balb/c Rag-2(−/−)Jak3(−/−) mice; however, the engraftment rate was far lower in C57/BL6 Rag-2(−/−)Jak3(−/−) mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment. Hindawi Publishing Corporation 2011 2011-01-26 /pmc/articles/PMC3035318/ /pubmed/21331358 http://dx.doi.org/10.1155/2011/539748 Text en Copyright © 2011 Ayumi Ono et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ono, Ayumi
Hattori, Shinichiro
Kariya, Ryusho
Iwanaga, Sumako
Taura, Manabu
Harada, Hideki
Suzu, Shinya
Okada, Seiji
Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title_full Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title_fullStr Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title_full_unstemmed Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title_short Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice
title_sort comparative study of human hematopoietic cell engraftment into balb/c and c57bl/6 strain of rag-2/jak3 double-deficient mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035318/
https://www.ncbi.nlm.nih.gov/pubmed/21331358
http://dx.doi.org/10.1155/2011/539748
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