Cargando…

Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer

A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U pla...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pasternak, Anna, Hernandez, Frank J., Rasmussen, Lars M., Vester, Birte, Wengel, Jesper
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035450/ https://www.ncbi.nlm.nih.gov/pubmed/20870750 http://dx.doi.org/10.1093/nar/gkq823

Ejemplares similares

Novel isoguanine derivative of unlocked nucleic acid—Investigations of thermodynamics and biological potential of modified thrombin binding aptamer
por: Kotkowiak, Weronika, et al.
Publicado: (2018)

Thermodynamics of RNA duplexes modified with unlocked nucleic acid nucleotides
por: Pasternak, Anna, et al.
Publicado: (2010)

G-rich VEGF aptamer with locked and unlocked nucleic acid modifications exhibits a unique G-quadruplex fold
por: Marušič, Maja, et al.
Publicado: (2013)

Conformationally rigid nucleoside probes help understand the role of sugar pucker and nucleobase orientation in the thrombin-binding aptamer
por: Saneyoshi, Hisao, et al.
Publicado: (2009)

Thermodynamic, Anticoagulant, and Antiproliferative Properties of Thrombin Binding Aptamer Containing Novel UNA Derivative
por: Kotkowiak, Weronika, et al.
Publicado: (2017)

Locked and Unlocked Nucleosides in Functional Nucleic Acids
por: Doessing, Holger, et al.
Publicado: (2011)

Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics
por: Kauppinen, Sakari, et al.
Publicado: (2005)

Redesign of the monomer–monomer interface of Cre recombinase yields an obligate heterotetrameric complex
por: Zhang, Chi, et al.
Publicado: (2015)

Systematic reconstruction of binding and stability landscapes of the fluorogenic aptamer spinach
por: Ketterer, Simon, et al.
Publicado: (2015)

A bridged nucleic acid, 2′,4′-BNA(COC): synthesis of fully modified oligonucleotides bearing thymine, 5-methylcytosine, adenine and guanine 2′,4′-BNA(COC) monomers and RNA-selective nucleic-acid recognition
por: Mitsuoka, Yasunori, et al.
Publicado: (2009)

Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
por: Carothers, James M., et al.
Publicado: (2010)

Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides
por: Højland, Torben, et al.
Publicado: (2012)

High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity
por: Russo Krauss, Irene, et al.
Publicado: (2012)

Exploiting natural riboswitches for aptamer engineering and validation
por: Mohsen, Michael G, et al.
Publicado: (2023)

Synthesis and structural characterization of piperazino-modified DNA that favours hybridization towards DNA over RNA
por: Skov, Joan, et al.
Publicado: (2011)

Formation of non-base-pairing DNA microgels using directed phase transition of amphiphilic monomers
por: Lee, Chanseok, et al.
Publicado: (2022)

Enhanced anti-HIV-1 activity of G-quadruplexes comprising locked nucleic acids and intercalating nucleic acids
por: Pedersen, Erik B., et al.
Publicado: (2011)

Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides
por: Kovačič, Matic, et al.
Publicado: (2020)

Development of an Efficient G‐Quadruplex‐Stabilised Thrombin‐Binding Aptamer Containing a Three‐Carbon Spacer Molecule
por: Aaldering, Lukas J., et al.
Publicado: (2017)

Dependence of aptamer activity on opposed terminal extensions: improvement of light-regulation efficiency
por: Buff, Maximilian C. R., et al.
Publicado: (2010)

A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia
por: Mallikaratchy, Prabodhika R., et al.
Publicado: (2011)

Iminodiacetic-phosphoramidates as metabolic prototypes for diversifying nucleic acid polymerization in vivo
por: Giraut, Anne, et al.
Publicado: (2010)

Prebiotically plausible mechanisms increase compositional diversity of nucleic acid sequences
por: Derr, Julien, et al.
Publicado: (2012)

Thrombin–aptamer recognition: a revealed ambiguity
por: Russo Krauss, Irene, et al.
Publicado: (2011)

A Comprehensive Analysis of the Thrombin Binding Aptamer Containing Functionalized Pyrrolo-2’-deoxycytidines
por: Kotkowiak, Weronika, et al.
Publicado: (2021)

A combinatorial approach to the repertoire of RNA kissing motifs; towards multiplex detection by switching hairpin aptamers
por: Durand, Guillaume, et al.
Publicado: (2016)

Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro
por: Shimo, Takenori, et al.
Publicado: (2014)

In silico direct folding of thrombin-binding aptamer G-quadruplex at all-atom level
por: Yang, Changwon, et al.
Publicado: (2017)

Base pair opening kinetics study of the aegPNA:DNA hydrid duplex containing a site-specific GNA-like chiral PNA monomer
por: Seo, Yeo-Jin, et al.
Publicado: (2011)

Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF(165) toward pharmaceutical applications
por: Kimoto, Michiko, et al.
Publicado: (2016)

Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
por: Vanmeert, Michiel, et al.
Publicado: (2019)

Optimizing anti-gene oligonucleotide ‘Zorro-LNA’ for improved strand invasion into duplex DNA
por: Zaghloul, Eman M., et al.
Publicado: (2011)

The immunorecognition, subcellular compartmentalization, and physicochemical properties of nucleic acid nanoparticles can be controlled by composition modification
por: Johnson, Morgan Brittany, et al.
Publicado: (2020)

High-affinity triplex targeting of double stranded DNA using chemically modified peptide nucleic acid oligomers
por: Hansen, Mads E., et al.
Publicado: (2009)

Structural transformation induced by locked nucleic acid or 2′–O-methyl nucleic acid site-specific modifications on thrombin binding aptamer
por: Liu, Bo, et al.
Publicado: (2014)

A synthetic snRNA m(3)G-CAP enhances nuclear delivery of exogenous proteins and nucleic acids
por: Moreno, Pedro M. D., et al.
Publicado: (2009)

Crystal structures of thrombin in complex with chemically modified thrombin DNA aptamers reveal the origins of enhanced affinity
por: Dolot, Rafal, et al.
Publicado: (2018)

Chemical synthesis, DNA incorporation and biological study of a new photocleavable 2′-deoxyadenosine mimic
por: Berthet, Nathalie, et al.
Publicado: (2009)

A new modified thrombin binding aptamer containing a 5′–5′ inversion of polarity site
por: Martino, Luigi, et al.
Publicado: (2006)

Selective recognition of parallel and anti-parallel thrombin-binding aptamer G-quadruplexes by different fluorescent dyes
por: Zhao, Dan, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_gnqghsva5ou6qskbfgutvsfbn4