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Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats

The aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Ad...

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Autores principales: Puzserova, Angelika, Bernatova, Iveta
Formato: Texto
Lenguaje:English
Publicado: Slovak Toxicology Society SETOX 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035566/
https://www.ncbi.nlm.nih.gov/pubmed/21331175
http://dx.doi.org/10.2478/v10102-010-0049-4
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author Puzserova, Angelika
Bernatova, Iveta
author_facet Puzserova, Angelika
Bernatova, Iveta
author_sort Puzserova, Angelika
collection PubMed
description The aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Adult male rats were randomly divided into control (living space: 480 cm(2)/rat) or stressed (living space: 200 cm(2)/rat) groups for 8 weeks. Blood pressure and heart rate, determined using tail-cuff plethysmography, were not influenced by stress vs. control. Conjugated dienes (CD) and concentrations of thiobarbituric acid-reactive substances (TBARS) were measured in the left ventricle and liver (for assessment of oxidative load) and were found unchanged by chronic crowding. The nitric oxide (NO)-dependent component of endothelium-dependent relaxation was investigated in the FA using a wire myograph. In both the presence and absence of AsA, acetylcholine-induced relaxation of the FA of stressed rats significantly exceeded that of the controls, which was associated with an increase of the NO-dependent component. In conclusion, the data showed that chronic crowding did not produce oxidative stress in the organs investigated and indicate that elevation of NO production during chronic stress is an important way of adaptation, which may prevent normotensive rats from the development of stress-induced hypertension.
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spelling pubmed-30355662011-02-17 Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats Puzserova, Angelika Bernatova, Iveta Interdiscip Toxicol Original Article The aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Adult male rats were randomly divided into control (living space: 480 cm(2)/rat) or stressed (living space: 200 cm(2)/rat) groups for 8 weeks. Blood pressure and heart rate, determined using tail-cuff plethysmography, were not influenced by stress vs. control. Conjugated dienes (CD) and concentrations of thiobarbituric acid-reactive substances (TBARS) were measured in the left ventricle and liver (for assessment of oxidative load) and were found unchanged by chronic crowding. The nitric oxide (NO)-dependent component of endothelium-dependent relaxation was investigated in the FA using a wire myograph. In both the presence and absence of AsA, acetylcholine-induced relaxation of the FA of stressed rats significantly exceeded that of the controls, which was associated with an increase of the NO-dependent component. In conclusion, the data showed that chronic crowding did not produce oxidative stress in the organs investigated and indicate that elevation of NO production during chronic stress is an important way of adaptation, which may prevent normotensive rats from the development of stress-induced hypertension. Slovak Toxicology Society SETOX 2010-12 2010-12 /pmc/articles/PMC3035566/ /pubmed/21331175 http://dx.doi.org/10.2478/v10102-010-0049-4 Text en Copyright © 2010 Slovak Toxicology Society SETOX http://creativecommons.org/licenses/by/2.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Puzserova, Angelika
Bernatova, Iveta
Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title_full Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title_fullStr Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title_full_unstemmed Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title_short Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
title_sort chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035566/
https://www.ncbi.nlm.nih.gov/pubmed/21331175
http://dx.doi.org/10.2478/v10102-010-0049-4
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