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Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron

Nwc represents a mysterious third evolutionarily conserved gene within the Rag locus. Here, we analyzed the phenotype of Nwc(tmpro1) mice, in which the Rag2 intragenic region containing the previously identified promoter responsible for initiating transcription of Nwc in all cells except lymphocytes...

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Autores principales: Laszkiewicz, Agnieszka, Cebrat, Malgorzata, Miazek, Arkadiusz, Kisielow, Pawel
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035778/
https://www.ncbi.nlm.nih.gov/pubmed/21243488
http://dx.doi.org/10.1007/s00251-011-0511-2
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author Laszkiewicz, Agnieszka
Cebrat, Malgorzata
Miazek, Arkadiusz
Kisielow, Pawel
author_facet Laszkiewicz, Agnieszka
Cebrat, Malgorzata
Miazek, Arkadiusz
Kisielow, Pawel
author_sort Laszkiewicz, Agnieszka
collection PubMed
description Nwc represents a mysterious third evolutionarily conserved gene within the Rag locus. Here, we analyzed the phenotype of Nwc(tmpro1) mice, in which the Rag2 intragenic region containing the previously identified promoter responsible for initiating transcription of Nwc in all cells except lymphocytes was deleted by homologous recombination. Despite strong nonlymphocyte-specific inhibition of Nwc transcription which runs through the regulatory region of Rag genes, their expression remained suppressed, and no developmental, morphological, anatomical, functional, physiological, or cellular defects in Nwc(tmpro1) mice could be observed. However, careful analysis of the Rag2 intergenic region uncovered a second evolutionarily conserved Nwc promoter region from which a previously unknown Nwc transcript can be generated in nonlymphocytes of Nwc(tmpro1) and normal mice. The above results reveal an unexpected additional complexity of transcriptional regulation within the Rag/Nwc locus and show that strong inhibition of Nwc transcription in nonlymphoid cells is well tolerated. Complete inactivation of Nwc is necessary to get insight into its function at transcriptional and posttranscriptional levels.
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spelling pubmed-30357782011-03-16 Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron Laszkiewicz, Agnieszka Cebrat, Malgorzata Miazek, Arkadiusz Kisielow, Pawel Immunogenetics Brief Communication Nwc represents a mysterious third evolutionarily conserved gene within the Rag locus. Here, we analyzed the phenotype of Nwc(tmpro1) mice, in which the Rag2 intragenic region containing the previously identified promoter responsible for initiating transcription of Nwc in all cells except lymphocytes was deleted by homologous recombination. Despite strong nonlymphocyte-specific inhibition of Nwc transcription which runs through the regulatory region of Rag genes, their expression remained suppressed, and no developmental, morphological, anatomical, functional, physiological, or cellular defects in Nwc(tmpro1) mice could be observed. However, careful analysis of the Rag2 intergenic region uncovered a second evolutionarily conserved Nwc promoter region from which a previously unknown Nwc transcript can be generated in nonlymphocytes of Nwc(tmpro1) and normal mice. The above results reveal an unexpected additional complexity of transcriptional regulation within the Rag/Nwc locus and show that strong inhibition of Nwc transcription in nonlymphoid cells is well tolerated. Complete inactivation of Nwc is necessary to get insight into its function at transcriptional and posttranscriptional levels. Springer-Verlag 2011-01-18 2011 /pmc/articles/PMC3035778/ /pubmed/21243488 http://dx.doi.org/10.1007/s00251-011-0511-2 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Brief Communication
Laszkiewicz, Agnieszka
Cebrat, Malgorzata
Miazek, Arkadiusz
Kisielow, Pawel
Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title_full Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title_fullStr Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title_full_unstemmed Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title_short Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron
title_sort complexity of transcriptional regulation within the rag locus: identification of a second nwc promoter region within the rag2 intron
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035778/
https://www.ncbi.nlm.nih.gov/pubmed/21243488
http://dx.doi.org/10.1007/s00251-011-0511-2
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