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Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity

The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reacti...

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Detalles Bibliográficos
Autores principales: Sakat, SS, Juvekar, AR
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035888/
https://www.ncbi.nlm.nih.gov/pubmed/21331194
http://dx.doi.org/10.4103/0975-1483.62216
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author Sakat, SS
Juvekar, AR
author_facet Sakat, SS
Juvekar, AR
author_sort Sakat, SS
collection PubMed
description The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids, and flavonols were estimated using the spectrophotometric method. Linear regression analysis was used to calculate the IC(50) value. Results showed that the aqueous and methanol extracts exhibited significant DPPH radicals scavenging activity with an IC(50) value 342.59 ± 19.59, 283.24 ± 12.28 µg/mL respectively. Nitric oxide radicals were significantly scavenged by the aqueous and methanol extracts (IC(50) = 250.12 ± 10.66; 328.29 ± 3.74 µg/mL). Lipid peroxidation induced by the Fe(2+) was inhibited by the aqueous extract with low IC(50) value (97.29 ± 2.05 µg/mL) as compared to methanol extract (IC(50) = 283.74 ± 5.70 µg/mL). Both the extracts were exhibited similar quantities of total phenolics. Total flavonoids were found to be in higher quantities than total flavonols in aqueous extract as compared to methanol extract. From the results, it is concluded that the aqueous and methanol extracts of E. indica leaves possesses significant antioxidant activity that may be due to the presence of flavonoids and related polyphenolic compounds.
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spelling pubmed-30358882011-02-17 Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity Sakat, SS Juvekar, AR J Young Pharm Pharmacology The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids, and flavonols were estimated using the spectrophotometric method. Linear regression analysis was used to calculate the IC(50) value. Results showed that the aqueous and methanol extracts exhibited significant DPPH radicals scavenging activity with an IC(50) value 342.59 ± 19.59, 283.24 ± 12.28 µg/mL respectively. Nitric oxide radicals were significantly scavenged by the aqueous and methanol extracts (IC(50) = 250.12 ± 10.66; 328.29 ± 3.74 µg/mL). Lipid peroxidation induced by the Fe(2+) was inhibited by the aqueous extract with low IC(50) value (97.29 ± 2.05 µg/mL) as compared to methanol extract (IC(50) = 283.74 ± 5.70 µg/mL). Both the extracts were exhibited similar quantities of total phenolics. Total flavonoids were found to be in higher quantities than total flavonols in aqueous extract as compared to methanol extract. From the results, it is concluded that the aqueous and methanol extracts of E. indica leaves possesses significant antioxidant activity that may be due to the presence of flavonoids and related polyphenolic compounds. Medknow Publications 2010 /pmc/articles/PMC3035888/ /pubmed/21331194 http://dx.doi.org/10.4103/0975-1483.62216 Text en © Journal of Young Pharmacists http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Pharmacology
Sakat, SS
Juvekar, AR
Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title_full Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title_fullStr Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title_full_unstemmed Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title_short Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity
title_sort comparative study of erythrina indica lam. (febaceae) leaves extracts for antioxidant activity
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035888/
https://www.ncbi.nlm.nih.gov/pubmed/21331194
http://dx.doi.org/10.4103/0975-1483.62216
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