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Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection

Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl(2), and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio P(Na)/P(...

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Autores principales: AOKI, Takaaki, HIRANO, Minako, TAKEUCHI, Yuko, KOBAYASHI, Toshihide, YANAGIDA, Toshio, IDE, Toru
Formato: Texto
Lenguaje:English
Publicado: The Japan Academy 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035922/
https://www.ncbi.nlm.nih.gov/pubmed/21084775
http://dx.doi.org/10.2183/pjab.86.920
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author AOKI, Takaaki
HIRANO, Minako
TAKEUCHI, Yuko
KOBAYASHI, Toshihide
YANAGIDA, Toshio
IDE, Toru
author_facet AOKI, Takaaki
HIRANO, Minako
TAKEUCHI, Yuko
KOBAYASHI, Toshihide
YANAGIDA, Toshio
IDE, Toru
author_sort AOKI, Takaaki
collection PubMed
description Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl(2), and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio P(Na)/P(Cl) was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations.
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spelling pubmed-30359222011-05-19 Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection AOKI, Takaaki HIRANO, Minako TAKEUCHI, Yuko KOBAYASHI, Toshihide YANAGIDA, Toshio IDE, Toru Proc Jpn Acad Ser B Phys Biol Sci Original Paper Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl(2), and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio P(Na)/P(Cl) was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations. The Japan Academy 2010-11-11 /pmc/articles/PMC3035922/ /pubmed/21084775 http://dx.doi.org/10.2183/pjab.86.920 Text en © 2010 The Japan Academy This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
AOKI, Takaaki
HIRANO, Minako
TAKEUCHI, Yuko
KOBAYASHI, Toshihide
YANAGIDA, Toshio
IDE, Toru
Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title_full Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title_fullStr Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title_full_unstemmed Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title_short Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
title_sort single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035922/
https://www.ncbi.nlm.nih.gov/pubmed/21084775
http://dx.doi.org/10.2183/pjab.86.920
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