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Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection

Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl(2), and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio P(Na)/P(...

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Detalles Bibliográficos
Autores principales: AOKI, Takaaki, HIRANO, Minako, TAKEUCHI, Yuko, KOBAYASHI, Toshihide, YANAGIDA, Toshio, IDE, Toru
Formato: Texto
Lenguaje:English
Publicado: The Japan Academy 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035922/
https://www.ncbi.nlm.nih.gov/pubmed/21084775
http://dx.doi.org/10.2183/pjab.86.920