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Transferability and characterization of microsatellite markers in two Neotropical Ficus species
Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 p...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036046/ https://www.ncbi.nlm.nih.gov/pubmed/21637521 http://dx.doi.org/10.1590/S1415-47572009005000056 |
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author | Nazareno, Alison Gonçalves Pereira, Rodrigo Augusto Santinelo Feres, Juliana Massimino Mestriner, Moacyr Antônio Alzate-Marin, Ana Lilia |
author_facet | Nazareno, Alison Gonçalves Pereira, Rodrigo Augusto Santinelo Feres, Juliana Massimino Mestriner, Moacyr Antônio Alzate-Marin, Ana Lilia |
author_sort | Nazareno, Alison Gonçalves |
collection | PubMed |
description | Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87. |
format | Text |
id | pubmed-3036046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-30360462011-06-02 Transferability and characterization of microsatellite markers in two Neotropical Ficus species Nazareno, Alison Gonçalves Pereira, Rodrigo Augusto Santinelo Feres, Juliana Massimino Mestriner, Moacyr Antônio Alzate-Marin, Ana Lilia Genet Mol Biol Plant Genetics Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87. Sociedade Brasileira de Genética 2009 2009-09-01 /pmc/articles/PMC3036046/ /pubmed/21637521 http://dx.doi.org/10.1590/S1415-47572009005000056 Text en Copyright © 2009, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Nazareno, Alison Gonçalves Pereira, Rodrigo Augusto Santinelo Feres, Juliana Massimino Mestriner, Moacyr Antônio Alzate-Marin, Ana Lilia Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title | Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title_full | Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title_fullStr | Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title_full_unstemmed | Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title_short | Transferability and characterization of microsatellite markers in two Neotropical Ficus species |
title_sort | transferability and characterization of microsatellite markers in two neotropical ficus species |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036046/ https://www.ncbi.nlm.nih.gov/pubmed/21637521 http://dx.doi.org/10.1590/S1415-47572009005000056 |
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