The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)

The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation...

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Autores principales: Mäder, Geraldo, Zamberlan, Priscilla M., Fagundes, Nelson J. R., Magnus, Tielli, Salzano, Francisco M., Bonatto, Sandro L., Freitas, Loreta B.
Formato: Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2010
Materias:
Plant Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036088/
https://www.ncbi.nlm.nih.gov/pubmed/21637612
http://dx.doi.org/10.1590/S1415-47572009005000101
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author Mäder, Geraldo
Zamberlan, Priscilla M.
Fagundes, Nelson J. R.
Magnus, Tielli
Salzano, Francisco M.
Bonatto, Sandro L.
Freitas, Loreta B.
author_facet Mäder, Geraldo
Zamberlan, Priscilla M.
Fagundes, Nelson J. R.
Magnus, Tielli
Salzano, Francisco M.
Bonatto, Sandro L.
Freitas, Loreta B.
author_sort Mäder, Geraldo
collection PubMed
description The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.
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spelling pubmed-30360882011-06-02 The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) Mäder, Geraldo Zamberlan, Priscilla M. Fagundes, Nelson J. R. Magnus, Tielli Salzano, Francisco M. Bonatto, Sandro L. Freitas, Loreta B. Genet Mol Biol Plant Genetics The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora. Sociedade Brasileira de Genética 2010 2010-03-01 /pmc/articles/PMC3036088/ /pubmed/21637612 http://dx.doi.org/10.1590/S1415-47572009005000101 Text en Copyright © 2010, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics
Mäder, Geraldo
Zamberlan, Priscilla M.
Fagundes, Nelson J. R.
Magnus, Tielli
Salzano, Francisco M.
Bonatto, Sandro L.
Freitas, Loreta B.
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_full The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_fullStr The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_full_unstemmed The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_short The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_sort use and limits of its data in the analysis of intraspecific variation in passiflora l. (passifloraceae)
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036088/
https://www.ncbi.nlm.nih.gov/pubmed/21637612
http://dx.doi.org/10.1590/S1415-47572009005000101
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