Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)

When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to...

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Detalles Bibliográficos
Autores principales: Tavares, Mara Garcia, Carvalho, Carlos Roberto, Soares, Fernanda Aparecida Ferrari, Fernandes, Anderson
Formato: Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2010
Materias:
Animal Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036126/
https://www.ncbi.nlm.nih.gov/pubmed/21637422
http://dx.doi.org/10.1590/S1415-47572010000300019
Descripción
Sumario:When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.

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