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Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut....
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Formato: | Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036135/ https://www.ncbi.nlm.nih.gov/pubmed/21637581 http://dx.doi.org/10.1590/S1415-47572010005000079 |
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author | Robledo, Germán Lavia, Graciela I. Seijo, Guillermo |
author_facet | Robledo, Germán Lavia, Graciela I. Seijo, Guillermo |
author_sort | Robledo, Germán |
collection | PubMed |
description | The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small “A chromosomes” characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. |
format | Text |
id | pubmed-3036135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-30361352011-06-02 Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut Robledo, Germán Lavia, Graciela I. Seijo, Guillermo Genet Mol Biol Plant Genetics The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small “A chromosomes” characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. Sociedade Brasileira de Genética 2010 2010-12-01 /pmc/articles/PMC3036135/ /pubmed/21637581 http://dx.doi.org/10.1590/S1415-47572010005000079 Text en Copyright © 2010, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Robledo, Germán Lavia, Graciela I. Seijo, Guillermo Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title | Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title_full | Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title_fullStr | Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title_full_unstemmed | Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title_short | Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut |
title_sort | genome re-assignment of arachis trinitensis (sect. arachis, leguminosae) and its implications for the genetic origin of cultivated peanut |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036135/ https://www.ncbi.nlm.nih.gov/pubmed/21637581 http://dx.doi.org/10.1590/S1415-47572010005000079 |
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