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Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes

BACKGROUND: The whitefly Trialeurodes vaporariorum is an economically important crop pest in temperate regions that has developed resistance to most classes of insecticides. However, the molecular mechanisms underlying resistance have not been characterised and, to date, progress has been hampered b...

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Autores principales: Karatolos, Nikos, Pauchet, Yannick, Wilkinson, Paul, Chauhan, Ritika, Denholm, Ian, Gorman, Kevin, Nelson, David R, Bass, Chris, ffrench-Constant, Richard H, Williamson, Martin S
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036619/
https://www.ncbi.nlm.nih.gov/pubmed/21261962
http://dx.doi.org/10.1186/1471-2164-12-56
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author Karatolos, Nikos
Pauchet, Yannick
Wilkinson, Paul
Chauhan, Ritika
Denholm, Ian
Gorman, Kevin
Nelson, David R
Bass, Chris
ffrench-Constant, Richard H
Williamson, Martin S
author_facet Karatolos, Nikos
Pauchet, Yannick
Wilkinson, Paul
Chauhan, Ritika
Denholm, Ian
Gorman, Kevin
Nelson, David R
Bass, Chris
ffrench-Constant, Richard H
Williamson, Martin S
author_sort Karatolos, Nikos
collection PubMed
description BACKGROUND: The whitefly Trialeurodes vaporariorum is an economically important crop pest in temperate regions that has developed resistance to most classes of insecticides. However, the molecular mechanisms underlying resistance have not been characterised and, to date, progress has been hampered by a lack of nucleotide sequence data for this species. Here, we use pyrosequencing on the Roche 454-FLX platform to produce a substantial and annotated EST dataset. This 'unigene set' will form a critical reference point for quantitation of over-expressed messages via digital transcriptomics. RESULTS: Pyrosequencing produced around a million sequencing reads that assembled into 54,748 contigs, with an average length of 965 bp, representing a dramatic expansion of existing cDNA sequences available for T. vaporariorum (only 43 entries in GenBank at the time of this publication). BLAST searching of non-redundant databases returned 20,333 significant matches and those gene families potentially encoding gene products involved in insecticide resistance were manually curated and annotated. These include, enzymes potentially involved in the detoxification of xenobiotics and those encoding the targets of the major chemical classes of insecticides. A total of 57 P450s, 17 GSTs and 27 CCEs were identified along with 30 contigs encoding the target proteins of six different insecticide classes. CONCLUSION: Here, we have developed new transcriptomic resources for T. vaporariorum. These include a substantial and annotated EST dataset that will serve the community studying this important crop pest and will elucidate further the molecular mechanisms underlying insecticide resistance.
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spelling pubmed-30366192011-02-10 Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes Karatolos, Nikos Pauchet, Yannick Wilkinson, Paul Chauhan, Ritika Denholm, Ian Gorman, Kevin Nelson, David R Bass, Chris ffrench-Constant, Richard H Williamson, Martin S BMC Genomics Research Article BACKGROUND: The whitefly Trialeurodes vaporariorum is an economically important crop pest in temperate regions that has developed resistance to most classes of insecticides. However, the molecular mechanisms underlying resistance have not been characterised and, to date, progress has been hampered by a lack of nucleotide sequence data for this species. Here, we use pyrosequencing on the Roche 454-FLX platform to produce a substantial and annotated EST dataset. This 'unigene set' will form a critical reference point for quantitation of over-expressed messages via digital transcriptomics. RESULTS: Pyrosequencing produced around a million sequencing reads that assembled into 54,748 contigs, with an average length of 965 bp, representing a dramatic expansion of existing cDNA sequences available for T. vaporariorum (only 43 entries in GenBank at the time of this publication). BLAST searching of non-redundant databases returned 20,333 significant matches and those gene families potentially encoding gene products involved in insecticide resistance were manually curated and annotated. These include, enzymes potentially involved in the detoxification of xenobiotics and those encoding the targets of the major chemical classes of insecticides. A total of 57 P450s, 17 GSTs and 27 CCEs were identified along with 30 contigs encoding the target proteins of six different insecticide classes. CONCLUSION: Here, we have developed new transcriptomic resources for T. vaporariorum. These include a substantial and annotated EST dataset that will serve the community studying this important crop pest and will elucidate further the molecular mechanisms underlying insecticide resistance. BioMed Central 2011-01-24 /pmc/articles/PMC3036619/ /pubmed/21261962 http://dx.doi.org/10.1186/1471-2164-12-56 Text en Copyright ©2011 Karatolos et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Karatolos, Nikos
Pauchet, Yannick
Wilkinson, Paul
Chauhan, Ritika
Denholm, Ian
Gorman, Kevin
Nelson, David R
Bass, Chris
ffrench-Constant, Richard H
Williamson, Martin S
Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title_full Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title_fullStr Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title_full_unstemmed Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title_short Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
title_sort pyrosequencing the transcriptome of the greenhouse whitefly, trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036619/
https://www.ncbi.nlm.nih.gov/pubmed/21261962
http://dx.doi.org/10.1186/1471-2164-12-56
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