Cargando…

Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin

The Karyopherin (Kap) family of nuclear transport receptors enables trafficking of proteins to and from the nucleus in a precise, regulated manner. Individual members function in overlapping pathways, while simultaneously being very specific for their main cargoes. The details of this apparent contr...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaves, Susana R., Rosenblum, Jonathan S.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036716/
https://www.ncbi.nlm.nih.gov/pubmed/21347375
http://dx.doi.org/10.1371/journal.pone.0016846
_version_ 1782197890574188544
author Chaves, Susana R.
Rosenblum, Jonathan S.
author_facet Chaves, Susana R.
Rosenblum, Jonathan S.
author_sort Chaves, Susana R.
collection PubMed
description The Karyopherin (Kap) family of nuclear transport receptors enables trafficking of proteins to and from the nucleus in a precise, regulated manner. Individual members function in overlapping pathways, while simultaneously being very specific for their main cargoes. The details of this apparent contradiction and rules governing pathway preference remain to be further elucidated. S. cerevisiae Lhp1 is an abundant protein that functions as an RNA chaperone in a variety of biologically important processes. It localizes almost exclusively to the nucleus and is imported by Kap108. We show that mutation of 3 of the 275 residues in Lhp1 alters its import pathway to a Kap121-dependent process. This mutant does not retain wild-type function and is bound by several chaperones. We propose that Kap121 also acts as a chaperone, one that can act as a genetic buffer by transporting mutated proteins to the nucleus.
format Text
id pubmed-3036716
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30367162011-02-23 Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin Chaves, Susana R. Rosenblum, Jonathan S. PLoS One Research Article The Karyopherin (Kap) family of nuclear transport receptors enables trafficking of proteins to and from the nucleus in a precise, regulated manner. Individual members function in overlapping pathways, while simultaneously being very specific for their main cargoes. The details of this apparent contradiction and rules governing pathway preference remain to be further elucidated. S. cerevisiae Lhp1 is an abundant protein that functions as an RNA chaperone in a variety of biologically important processes. It localizes almost exclusively to the nucleus and is imported by Kap108. We show that mutation of 3 of the 275 residues in Lhp1 alters its import pathway to a Kap121-dependent process. This mutant does not retain wild-type function and is bound by several chaperones. We propose that Kap121 also acts as a chaperone, one that can act as a genetic buffer by transporting mutated proteins to the nucleus. Public Library of Science 2011-02-09 /pmc/articles/PMC3036716/ /pubmed/21347375 http://dx.doi.org/10.1371/journal.pone.0016846 Text en Chaves, Rosenblum. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chaves, Susana R.
Rosenblum, Jonathan S.
Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title_full Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title_fullStr Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title_full_unstemmed Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title_short Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin
title_sort efficient nuclear transport of structurally disturbed cargo: mutations in a cargo protein switch its cognate karyopherin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036716/
https://www.ncbi.nlm.nih.gov/pubmed/21347375
http://dx.doi.org/10.1371/journal.pone.0016846
work_keys_str_mv AT chavessusanar efficientnucleartransportofstructurallydisturbedcargomutationsinacargoproteinswitchitscognatekaryopherin
AT rosenblumjonathans efficientnucleartransportofstructurallydisturbedcargomutationsinacargoproteinswitchitscognatekaryopherin