Cargando…

Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin

Dynactin is a multisubunit protein complex required for the activity of cytoplasmic dynein. In Caenorhabditis elegans, although 10 of the 11 dynactin subunits were identified based on the sequence similarities to their orthologs, the p24/p22 subunit has not been detected in the genome. Here, we demo...

Descripción completa

Detalles Bibliográficos
Autores principales: Terasawa, Masahiro, Toya, Mika, Motegi, Fumio, Mana, Miyeko, Nakamura, Kuniaki, Sugimoto, Asako
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036819/
https://www.ncbi.nlm.nih.gov/pubmed/20964796
http://dx.doi.org/10.1111/j.1365-2443.2010.01451.x
_version_ 1782197903523053568
author Terasawa, Masahiro
Toya, Mika
Motegi, Fumio
Mana, Miyeko
Nakamura, Kuniaki
Sugimoto, Asako
author_facet Terasawa, Masahiro
Toya, Mika
Motegi, Fumio
Mana, Miyeko
Nakamura, Kuniaki
Sugimoto, Asako
author_sort Terasawa, Masahiro
collection PubMed
description Dynactin is a multisubunit protein complex required for the activity of cytoplasmic dynein. In Caenorhabditis elegans, although 10 of the 11 dynactin subunits were identified based on the sequence similarities to their orthologs, the p24/p22 subunit has not been detected in the genome. Here, we demonstrate that DNC-3 (W10G11.20) is the functional counterpart of the p24/p22 subunit in C. elegans. RNAi phenotypes and subcellular localization of DNC-3 in early C. elegans embryos were nearly identical to those of the known dynactin components. All other dynactin subunits were co-immunoprecipitated with DNC-3, indicating that DNC-3 is a core component of dynactin. Furthermore, the overall secondary structure of DNC-3 resembles to those of the mammalian and yeast p24/p22. We found that DNC-3 is required for the localization of the DNC-1/p150(Glued) and DNC-2/dynamitin, the two components of the projection arm of dynactin, to the nuclear envelope of meiotic nuclei in the adult gonad. Moreover, DNC-3 physically interacted with DNC-1 and DNC-2 and significantly enhanced the binding ability between DNC-1 and DNC-2 in vitro. These results suggest that DNC-3 is essential for the formation of the projection arm subcomplex of dynactin.
format Text
id pubmed-3036819
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-30368192011-02-15 Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin Terasawa, Masahiro Toya, Mika Motegi, Fumio Mana, Miyeko Nakamura, Kuniaki Sugimoto, Asako Genes Cells Original Articles Dynactin is a multisubunit protein complex required for the activity of cytoplasmic dynein. In Caenorhabditis elegans, although 10 of the 11 dynactin subunits were identified based on the sequence similarities to their orthologs, the p24/p22 subunit has not been detected in the genome. Here, we demonstrate that DNC-3 (W10G11.20) is the functional counterpart of the p24/p22 subunit in C. elegans. RNAi phenotypes and subcellular localization of DNC-3 in early C. elegans embryos were nearly identical to those of the known dynactin components. All other dynactin subunits were co-immunoprecipitated with DNC-3, indicating that DNC-3 is a core component of dynactin. Furthermore, the overall secondary structure of DNC-3 resembles to those of the mammalian and yeast p24/p22. We found that DNC-3 is required for the localization of the DNC-1/p150(Glued) and DNC-2/dynamitin, the two components of the projection arm of dynactin, to the nuclear envelope of meiotic nuclei in the adult gonad. Moreover, DNC-3 physically interacted with DNC-1 and DNC-2 and significantly enhanced the binding ability between DNC-1 and DNC-2 in vitro. These results suggest that DNC-3 is essential for the formation of the projection arm subcomplex of dynactin. Blackwell Publishing Ltd 2010-11 /pmc/articles/PMC3036819/ /pubmed/20964796 http://dx.doi.org/10.1111/j.1365-2443.2010.01451.x Text en Journal compilation © 2010 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
Terasawa, Masahiro
Toya, Mika
Motegi, Fumio
Mana, Miyeko
Nakamura, Kuniaki
Sugimoto, Asako
Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title_full Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title_fullStr Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title_full_unstemmed Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title_short Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin
title_sort caenorhabditis elegans ortholog of the p24/p22 subunit, dnc-3, is essential for the formation of the dynactin complex by bridging dnc-1/p150(glued) and dnc-2/dynamitin
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036819/
https://www.ncbi.nlm.nih.gov/pubmed/20964796
http://dx.doi.org/10.1111/j.1365-2443.2010.01451.x
work_keys_str_mv AT terasawamasahiro caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin
AT toyamika caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin
AT motegifumio caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin
AT manamiyeko caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin
AT nakamurakuniaki caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin
AT sugimotoasako caenorhabditiselegansorthologofthep24p22subunitdnc3isessentialfortheformationofthedynactincomplexbybridgingdnc1p150gluedanddnc2dynamitin