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Recombinase technology: applications and possibilities
The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087–2096, 1987). The random insertion of a transgene into...
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036822/ https://www.ncbi.nlm.nih.gov/pubmed/20972794 http://dx.doi.org/10.1007/s00299-010-0938-1 |
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author | Wang, Yueju Yau, Yuan-Yeu Perkins-Balding, Donna Thomson, James G. |
author_facet | Wang, Yueju Yau, Yuan-Yeu Perkins-Balding, Donna Thomson, James G. |
author_sort | Wang, Yueju |
collection | PubMed |
description | The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087–2096, 1987). The random insertion of a transgene into a plant genome by traditional methods generates unpredictable expression patterns. This feature of transgenesis makes screening for functional lines with predictable expression labor intensive and time consuming. Furthermore, an antibiotic resistance gene is often left in the final product and the potential escape of such resistance markers into the environment and their potential consumption raises consumer concern. The use of site-specific recombination technology in plant genome manipulation has been demonstrated to effectively resolve complex transgene insertions to single copy, remove unwanted DNA, and precisely insert DNA into known genomic target sites. Recombinases have also been demonstrated capable of site-specific recombination within non-nuclear targets, such as the plastid genome of tobacco. Here, we review multiple uses of site-specific recombination and their application toward plant genomic engineering. We also provide alternative strategies for the combined use of multiple site-specific recombinase systems for genome engineering to precisely insert transgenes into a pre-determined locus, and removal of unwanted selectable marker genes. |
format | Text |
id | pubmed-3036822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30368222011-03-16 Recombinase technology: applications and possibilities Wang, Yueju Yau, Yuan-Yeu Perkins-Balding, Donna Thomson, James G. Plant Cell Rep Review The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087–2096, 1987). The random insertion of a transgene into a plant genome by traditional methods generates unpredictable expression patterns. This feature of transgenesis makes screening for functional lines with predictable expression labor intensive and time consuming. Furthermore, an antibiotic resistance gene is often left in the final product and the potential escape of such resistance markers into the environment and their potential consumption raises consumer concern. The use of site-specific recombination technology in plant genome manipulation has been demonstrated to effectively resolve complex transgene insertions to single copy, remove unwanted DNA, and precisely insert DNA into known genomic target sites. Recombinases have also been demonstrated capable of site-specific recombination within non-nuclear targets, such as the plastid genome of tobacco. Here, we review multiple uses of site-specific recombination and their application toward plant genomic engineering. We also provide alternative strategies for the combined use of multiple site-specific recombinase systems for genome engineering to precisely insert transgenes into a pre-determined locus, and removal of unwanted selectable marker genes. Springer-Verlag 2010-10-24 2011 /pmc/articles/PMC3036822/ /pubmed/20972794 http://dx.doi.org/10.1007/s00299-010-0938-1 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Review Wang, Yueju Yau, Yuan-Yeu Perkins-Balding, Donna Thomson, James G. Recombinase technology: applications and possibilities |
title | Recombinase technology: applications and possibilities |
title_full | Recombinase technology: applications and possibilities |
title_fullStr | Recombinase technology: applications and possibilities |
title_full_unstemmed | Recombinase technology: applications and possibilities |
title_short | Recombinase technology: applications and possibilities |
title_sort | recombinase technology: applications and possibilities |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036822/ https://www.ncbi.nlm.nih.gov/pubmed/20972794 http://dx.doi.org/10.1007/s00299-010-0938-1 |
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