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Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers
Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic des...
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Formato: | Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036869/ https://www.ncbi.nlm.nih.gov/pubmed/21637487 http://dx.doi.org/10.1590/S1415-47572010005000039 |
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author | Gouvêa, Lígia Regina Lima Rubiano, Luciana Benchimol Chioratto, Alisson Fernando Zucchi, Maria Imaculada de Souza Gonçalves, Paulo |
author_facet | Gouvêa, Lígia Regina Lima Rubiano, Luciana Benchimol Chioratto, Alisson Fernando Zucchi, Maria Imaculada de Souza Gonçalves, Paulo |
author_sort | Gouvêa, Lígia Regina Lima |
collection | PubMed |
description | Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered into 12 groups according to the Tocher method, while the molecular analysis identified six groups. In the phenotypic and microsatellite analyses, the Amazonian and IAC genotypes were distributed in several groups, whereas the Asiatic were in only a few. Observed heterozygosity ranged from 0.05 to 0.96. Both high total diversity (H(T') = 0.58) and high gene differentiation (G (st') = 0.61) were observed, and indicated high genetic variation among the 60 genotypes, which may be useful for breeding programs. The analyzed agronomic parameters and SSRs markers were effective in assessing genetic diversity among Hevea genotypes, besides proving to be useful for characterizing genetic variability. |
format | Text |
id | pubmed-3036869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-30368692011-06-02 Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers Gouvêa, Lígia Regina Lima Rubiano, Luciana Benchimol Chioratto, Alisson Fernando Zucchi, Maria Imaculada de Souza Gonçalves, Paulo Genet Mol Biol Plant Genetics Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered into 12 groups according to the Tocher method, while the molecular analysis identified six groups. In the phenotypic and microsatellite analyses, the Amazonian and IAC genotypes were distributed in several groups, whereas the Asiatic were in only a few. Observed heterozygosity ranged from 0.05 to 0.96. Both high total diversity (H(T') = 0.58) and high gene differentiation (G (st') = 0.61) were observed, and indicated high genetic variation among the 60 genotypes, which may be useful for breeding programs. The analyzed agronomic parameters and SSRs markers were effective in assessing genetic diversity among Hevea genotypes, besides proving to be useful for characterizing genetic variability. Sociedade Brasileira de Genética 2010 2010-06-01 /pmc/articles/PMC3036869/ /pubmed/21637487 http://dx.doi.org/10.1590/S1415-47572010005000039 Text en Copyright © 2010, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Gouvêa, Lígia Regina Lima Rubiano, Luciana Benchimol Chioratto, Alisson Fernando Zucchi, Maria Imaculada de Souza Gonçalves, Paulo Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title | Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title_full | Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title_fullStr | Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title_full_unstemmed | Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title_short | Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
title_sort | genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036869/ https://www.ncbi.nlm.nih.gov/pubmed/21637487 http://dx.doi.org/10.1590/S1415-47572010005000039 |
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