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Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex

Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B.setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical te...

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Autores principales: García, Ana M., Schrauf, Gustavo E., González, Graciela, Poggio, Lidia, Naranjo, Carlos A., Dupal, Marck P., Spangenberg, Germán C., Forster, John W.
Formato: Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036919/
https://www.ncbi.nlm.nih.gov/pubmed/21637686
http://dx.doi.org/10.1590/S1415-47572009005000029
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author García, Ana M.
Schrauf, Gustavo E.
González, Graciela
Poggio, Lidia
Naranjo, Carlos A.
Dupal, Marck P.
Spangenberg, Germán C.
Forster, John W.
author_facet García, Ana M.
Schrauf, Gustavo E.
González, Graciela
Poggio, Lidia
Naranjo, Carlos A.
Dupal, Marck P.
Spangenberg, Germán C.
Forster, John W.
author_sort García, Ana M.
collection PubMed
description Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B.setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of molecular genetic and cytogenetic evidence supported a species status for two of the three taxa and suggested hypotheses for the evolutionary origin of these complex taxa. Species status was also indicated for B. setifolius var. setifolius. Based on these findings, we suggest that B. setifolius var. pictus be referred to as B. pictus Hook var. pictus, and B. setifolius var brevifolius as B. pictus Hook var brevifolius. The correlation between AFLP diversity and variation in ecological parameters suggested that this marker system could be used to assess breeding progress and to monitor the domestication of Patagonian Bromus species for agronomic use.
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spelling pubmed-30369192011-06-02 Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex García, Ana M. Schrauf, Gustavo E. González, Graciela Poggio, Lidia Naranjo, Carlos A. Dupal, Marck P. Spangenberg, Germán C. Forster, John W. Genet Mol Biol Plant Genetics Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B.setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of molecular genetic and cytogenetic evidence supported a species status for two of the three taxa and suggested hypotheses for the evolutionary origin of these complex taxa. Species status was also indicated for B. setifolius var. setifolius. Based on these findings, we suggest that B. setifolius var. pictus be referred to as B. pictus Hook var. pictus, and B. setifolius var brevifolius as B. pictus Hook var brevifolius. The correlation between AFLP diversity and variation in ecological parameters suggested that this marker system could be used to assess breeding progress and to monitor the domestication of Patagonian Bromus species for agronomic use. Sociedade Brasileira de Genética 2009 2009-06-01 /pmc/articles/PMC3036919/ /pubmed/21637686 http://dx.doi.org/10.1590/S1415-47572009005000029 Text en Copyright © 2009, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics
García, Ana M.
Schrauf, Gustavo E.
González, Graciela
Poggio, Lidia
Naranjo, Carlos A.
Dupal, Marck P.
Spangenberg, Germán C.
Forster, John W.
Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title_full Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title_fullStr Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title_full_unstemmed Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title_short Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
title_sort use of aflp and rapd molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the patagonian bromus setifolius complex
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036919/
https://www.ncbi.nlm.nih.gov/pubmed/21637686
http://dx.doi.org/10.1590/S1415-47572009005000029
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