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Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH
The karyotypes of four South American species of Cestrum (C. capsulare,C. corymbosum,C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metace...
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Formato: | Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036934/ https://www.ncbi.nlm.nih.gov/pubmed/21637687 http://dx.doi.org/10.1590/S1415-47572009000200019 |
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author | Fernandes, Thiago de Almeida Rego, Letícia do Nascimento Andrade Nardy, Mariana Yuyama, Priscila Mary Vanzela, André Luís Laforga |
author_facet | Fernandes, Thiago de Almeida Rego, Letícia do Nascimento Andrade Nardy, Mariana Yuyama, Priscila Mary Vanzela, André Luís Laforga |
author_sort | Fernandes, Thiago |
collection | PubMed |
description | The karyotypes of four South American species of Cestrum (C. capsulare,C. corymbosum,C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA(+) bands and 45S rDNA were located predominantly in terminal regions. The C-CMA (+) /DAPI (+) bands appeared in interstitial and terminal regions, and the C-DAPI (+) bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species except C. capsulare, where they were found in the paracentromeric region of the long arm of pair 4. The differences in band patterns among the species studied here, along with data from other nine species reported in the literature, suggest that the bands are dispersed in an equilocal and non-equilocal manner and that structural rearrangements can be responsible for internal karyotype diversification. However, it is important to point out that the structural changes involving repetitive segments did not culminate in substantial changes in the general karyotype structure concerning chromosome size and morphology. |
format | Text |
id | pubmed-3036934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-30369342011-06-02 Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH Fernandes, Thiago de Almeida Rego, Letícia do Nascimento Andrade Nardy, Mariana Yuyama, Priscila Mary Vanzela, André Luís Laforga Genet Mol Biol Plant Genetics The karyotypes of four South American species of Cestrum (C. capsulare,C. corymbosum,C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA(+) bands and 45S rDNA were located predominantly in terminal regions. The C-CMA (+) /DAPI (+) bands appeared in interstitial and terminal regions, and the C-DAPI (+) bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species except C. capsulare, where they were found in the paracentromeric region of the long arm of pair 4. The differences in band patterns among the species studied here, along with data from other nine species reported in the literature, suggest that the bands are dispersed in an equilocal and non-equilocal manner and that structural rearrangements can be responsible for internal karyotype diversification. However, it is important to point out that the structural changes involving repetitive segments did not culminate in substantial changes in the general karyotype structure concerning chromosome size and morphology. Sociedade Brasileira de Genética 2009 2009-06-01 /pmc/articles/PMC3036934/ /pubmed/21637687 http://dx.doi.org/10.1590/S1415-47572009000200019 Text en Copyright © 2009, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Fernandes, Thiago de Almeida Rego, Letícia do Nascimento Andrade Nardy, Mariana Yuyama, Priscila Mary Vanzela, André Luís Laforga Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title | Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title_full | Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title_fullStr | Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title_full_unstemmed | Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title_short | Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH |
title_sort | karyotype differentiation of four cestrum species (solanaceae) revealed by fluorescent chromosome banding and fish |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036934/ https://www.ncbi.nlm.nih.gov/pubmed/21637687 http://dx.doi.org/10.1590/S1415-47572009000200019 |
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