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How and Why DNA Barcodes Underestimate the Diversity of Microbial Eukaryotes

BACKGROUND: Because many picoplanktonic eukaryotic species cannot currently be maintained in culture, direct sequencing of PCR-amplified 18S ribosomal gene DNA fragments from filtered sea-water has been successfully used to investigate the astounding diversity of these organisms. The recognition of...

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Detalles Bibliográficos
Autores principales: Piganeau, Gwenael, Eyre-Walker, Adam, Grimsley, Nigel, Moreau, Hervé
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037371/
https://www.ncbi.nlm.nih.gov/pubmed/21347361
http://dx.doi.org/10.1371/journal.pone.0016342