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Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease

Conventional leather processing involving depilation of animal hide by lime and sulphide treatment generates considerable amounts of chemical waste causing severe environmental pollution. Enzymatic depilation is an environmentally friendly process and has been considered to be a viable alternative t...

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Autores principales: Pandeeti, Emmanuel Vijay Paul, Pitchika, Gopi Krishna, Jotshi, Jyotsna, Nilegaonkar, Smita S., Kanekar, Pradnya P., Siddavattam, Dayananda
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037957/
https://www.ncbi.nlm.nih.gov/pubmed/21347249
http://dx.doi.org/10.1371/journal.pone.0016742
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author Pandeeti, Emmanuel Vijay Paul
Pitchika, Gopi Krishna
Jotshi, Jyotsna
Nilegaonkar, Smita S.
Kanekar, Pradnya P.
Siddavattam, Dayananda
author_facet Pandeeti, Emmanuel Vijay Paul
Pitchika, Gopi Krishna
Jotshi, Jyotsna
Nilegaonkar, Smita S.
Kanekar, Pradnya P.
Siddavattam, Dayananda
author_sort Pandeeti, Emmanuel Vijay Paul
collection PubMed
description Conventional leather processing involving depilation of animal hide by lime and sulphide treatment generates considerable amounts of chemical waste causing severe environmental pollution. Enzymatic depilation is an environmentally friendly process and has been considered to be a viable alternative to the chemical depilation process. We isolated an extracellular protease from Pseudomonas aeruginosa strain MCM B-327 with high depilation activity using buffalo hide as a substrate. This 33 kDa protease generated a peptide mass fingerprint and de novo sequence that matched perfectly with LasB (elastase), of Pseudomonas aeruginosa. In support of this data a lasB mutant of MCM B-327 strain lacked depilatory activity and failed to produce LasB. LasB heterologously over-produced and purified from Escherichia coli also exhibited high depilating activity. Moreover, reintroduction of the lasB gene to the P. aeruginosa lasB mutant via a knock-in strategy also successfully restored depilation activity thus confirming the role of LasB as the depilating enzyme.
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spelling pubmed-30379572011-02-23 Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease Pandeeti, Emmanuel Vijay Paul Pitchika, Gopi Krishna Jotshi, Jyotsna Nilegaonkar, Smita S. Kanekar, Pradnya P. Siddavattam, Dayananda PLoS One Research Article Conventional leather processing involving depilation of animal hide by lime and sulphide treatment generates considerable amounts of chemical waste causing severe environmental pollution. Enzymatic depilation is an environmentally friendly process and has been considered to be a viable alternative to the chemical depilation process. We isolated an extracellular protease from Pseudomonas aeruginosa strain MCM B-327 with high depilation activity using buffalo hide as a substrate. This 33 kDa protease generated a peptide mass fingerprint and de novo sequence that matched perfectly with LasB (elastase), of Pseudomonas aeruginosa. In support of this data a lasB mutant of MCM B-327 strain lacked depilatory activity and failed to produce LasB. LasB heterologously over-produced and purified from Escherichia coli also exhibited high depilating activity. Moreover, reintroduction of the lasB gene to the P. aeruginosa lasB mutant via a knock-in strategy also successfully restored depilation activity thus confirming the role of LasB as the depilating enzyme. Public Library of Science 2011-02-11 /pmc/articles/PMC3037957/ /pubmed/21347249 http://dx.doi.org/10.1371/journal.pone.0016742 Text en Pandeeti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pandeeti, Emmanuel Vijay Paul
Pitchika, Gopi Krishna
Jotshi, Jyotsna
Nilegaonkar, Smita S.
Kanekar, Pradnya P.
Siddavattam, Dayananda
Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title_full Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title_fullStr Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title_full_unstemmed Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title_short Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease
title_sort enzymatic depilation of animal hide: identification of elastase (lasb) from pseudomonas aeruginosa mcm b-327 as a depilating protease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037957/
https://www.ncbi.nlm.nih.gov/pubmed/21347249
http://dx.doi.org/10.1371/journal.pone.0016742
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