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Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response

Human onchocerciasis, also known as River Blindness, is a debilitating disease caused by the filarial nematode Onchocerca volvulus. Many, but not all, filarial nematodes carry within their tissues endosymbiotic, Rickettsia-like bacteria of the genus Wolbachia. Onchocerca spp. infections in cattle of...

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Autores principales: Neary, Joseph M., Trees, Alexander J., Ekale, David D., Tanya, Vincent N., Hetzel, Udo, Makepeace, Benjamin L.
Formato: Texto
Lenguaje:English
Publicado: Elsevier 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038270/
https://www.ncbi.nlm.nih.gov/pubmed/20850932
http://dx.doi.org/10.1016/j.vetpar.2010.08.031
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author Neary, Joseph M.
Trees, Alexander J.
Ekale, David D.
Tanya, Vincent N.
Hetzel, Udo
Makepeace, Benjamin L.
author_facet Neary, Joseph M.
Trees, Alexander J.
Ekale, David D.
Tanya, Vincent N.
Hetzel, Udo
Makepeace, Benjamin L.
author_sort Neary, Joseph M.
collection PubMed
description Human onchocerciasis, also known as River Blindness, is a debilitating disease caused by the filarial nematode Onchocerca volvulus. Many, but not all, filarial nematodes carry within their tissues endosymbiotic, Rickettsia-like bacteria of the genus Wolbachia. Onchocerca spp. infections in cattle offer the most relevant, analogous host–parasite model system. West African cattle are commonly co-infected with four Onchocerca spp.; two of these are Wolbachia-positive (Onchocerca gutturosa and Onchocerca ochengi), and the remainder are of unknown Wolbachia status (Onchocerca dukei and Onchocerca armillata). Previous studies have suggested that worm survival is dependent on this bacterium. O. armillata, an abundant parasite of African cattle that has received little attention, is a primitive species that may lack Wolbachia. The objectives of this study were to determine if O. armillata carries Wolbachia and to provide preliminary descriptions of the host inflammatory cell environment around the adult worms. The findings may support or refute the hypothesis that a prime contribution of Wolbachia is to permit long-term survival and reproduction of certain Onchocerca spp. (including O. volvulus in humans). O. armillata adult worms were found in the aorta of 90.7% of cattle (n = 54) slaughtered at an abattoir in Ngaoundéré, Adamawa Region, Cameroon. The presence of Wolbachia in O. armillata was confirmed by a specific anti-Wolbachia surface protein antibody detected using a peroxidase conjugate (immunohistochemistry) and PCR for detection of Wolbachia-specific sequences within DNA extracts from frozen worms. Tissue sections stained with haematoxylin and eosin showed the host cell response to be dominated by macrophages and fibroblasts. This is unusual compared with nodule-dwelling Wolbachia-positive Onchocerca spp., where the host response is typically characterised by granulocytes, and suggests that the mechanisms for worm survival employed by this species (which is probably motile) may differ.
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spelling pubmed-30382702011-03-14 Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response Neary, Joseph M. Trees, Alexander J. Ekale, David D. Tanya, Vincent N. Hetzel, Udo Makepeace, Benjamin L. Vet Parasitol Article Human onchocerciasis, also known as River Blindness, is a debilitating disease caused by the filarial nematode Onchocerca volvulus. Many, but not all, filarial nematodes carry within their tissues endosymbiotic, Rickettsia-like bacteria of the genus Wolbachia. Onchocerca spp. infections in cattle offer the most relevant, analogous host–parasite model system. West African cattle are commonly co-infected with four Onchocerca spp.; two of these are Wolbachia-positive (Onchocerca gutturosa and Onchocerca ochengi), and the remainder are of unknown Wolbachia status (Onchocerca dukei and Onchocerca armillata). Previous studies have suggested that worm survival is dependent on this bacterium. O. armillata, an abundant parasite of African cattle that has received little attention, is a primitive species that may lack Wolbachia. The objectives of this study were to determine if O. armillata carries Wolbachia and to provide preliminary descriptions of the host inflammatory cell environment around the adult worms. The findings may support or refute the hypothesis that a prime contribution of Wolbachia is to permit long-term survival and reproduction of certain Onchocerca spp. (including O. volvulus in humans). O. armillata adult worms were found in the aorta of 90.7% of cattle (n = 54) slaughtered at an abattoir in Ngaoundéré, Adamawa Region, Cameroon. The presence of Wolbachia in O. armillata was confirmed by a specific anti-Wolbachia surface protein antibody detected using a peroxidase conjugate (immunohistochemistry) and PCR for detection of Wolbachia-specific sequences within DNA extracts from frozen worms. Tissue sections stained with haematoxylin and eosin showed the host cell response to be dominated by macrophages and fibroblasts. This is unusual compared with nodule-dwelling Wolbachia-positive Onchocerca spp., where the host response is typically characterised by granulocytes, and suggests that the mechanisms for worm survival employed by this species (which is probably motile) may differ. Elsevier 2010-12-15 /pmc/articles/PMC3038270/ /pubmed/20850932 http://dx.doi.org/10.1016/j.vetpar.2010.08.031 Text en © 2010 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Neary, Joseph M.
Trees, Alexander J.
Ekale, David D.
Tanya, Vincent N.
Hetzel, Udo
Makepeace, Benjamin L.
Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title_full Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title_fullStr Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title_full_unstemmed Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title_short Onchocerca armillata contains the endosymbiotic bacterium Wolbachia and elicits a limited inflammatory response
title_sort onchocerca armillata contains the endosymbiotic bacterium wolbachia and elicits a limited inflammatory response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038270/
https://www.ncbi.nlm.nih.gov/pubmed/20850932
http://dx.doi.org/10.1016/j.vetpar.2010.08.031
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