Superresolved femtosecond laser nanosurgery of cells

We report on femtosecond nanosurgery of fluorescently labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced using phase filtering applied with a programmable phase modulator. A comprehensive statistical analysis of the resulting cuts demonstrates an a...

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Detalles Bibliográficos
Autores principales: Pospiech, Matthias, Emons, Moritz, Kuetemeyer, Kai, Heisterkamp, Alexander, Morgner, Uwe
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038442/
https://www.ncbi.nlm.nih.gov/pubmed/21339872
http://dx.doi.org/10.1364/BOE.1.000264
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author Pospiech, Matthias
Emons, Moritz
Kuetemeyer, Kai
Heisterkamp, Alexander
Morgner, Uwe
author_facet Pospiech, Matthias
Emons, Moritz
Kuetemeyer, Kai
Heisterkamp, Alexander
Morgner, Uwe
author_sort Pospiech, Matthias
collection PubMed
description We report on femtosecond nanosurgery of fluorescently labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced using phase filtering applied with a programmable phase modulator. A comprehensive statistical analysis of the resulting cuts demonstrates an achievable average resolution enhancement of 30 %.
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spelling pubmed-30384422011-02-18 Superresolved femtosecond laser nanosurgery of cells Pospiech, Matthias Emons, Moritz Kuetemeyer, Kai Heisterkamp, Alexander Morgner, Uwe Biomed Opt Express Microscopy We report on femtosecond nanosurgery of fluorescently labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced using phase filtering applied with a programmable phase modulator. A comprehensive statistical analysis of the resulting cuts demonstrates an achievable average resolution enhancement of 30 %. Optical Society of America 2011-01-05 /pmc/articles/PMC3038442/ /pubmed/21339872 http://dx.doi.org/10.1364/BOE.1.000264 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Microscopy
Pospiech, Matthias
Emons, Moritz
Kuetemeyer, Kai
Heisterkamp, Alexander
Morgner, Uwe
Superresolved femtosecond laser nanosurgery of cells
title Superresolved femtosecond laser nanosurgery of cells
title_full Superresolved femtosecond laser nanosurgery of cells
title_fullStr Superresolved femtosecond laser nanosurgery of cells
title_full_unstemmed Superresolved femtosecond laser nanosurgery of cells
title_short Superresolved femtosecond laser nanosurgery of cells
title_sort superresolved femtosecond laser nanosurgery of cells
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038442/
https://www.ncbi.nlm.nih.gov/pubmed/21339872
http://dx.doi.org/10.1364/BOE.1.000264
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AT kuetemeyerkai superresolvedfemtosecondlasernanosurgeryofcells
AT heisterkampalexander superresolvedfemtosecondlasernanosurgeryofcells
AT morgneruwe superresolvedfemtosecondlasernanosurgeryofcells

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