Multimodal wide-field two-photon excitation imaging: characterization of the technique for in vivo applications

We report fast, non-scanning, wide-field two-photon fluorescence excitation with spectral and lifetime detection for in vivo biomedical applications. We determined the optical characteristics of the technique, developed a Gaussian flat-field correction method to reduce artifacts resulting from non-u...

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Detalles Bibliográficos
Autores principales: Hwang, Jae Youn, Wachsmann-Hogiu, Sebastian, Ramanujan, V Krishnan, Nowatzyk, Andreas G., Koronyo, Yosef, Medina-Kauwe, Lali K., Gross, Zeev, Gray, Harry B., Farkas, Daniel L.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038450/
https://www.ncbi.nlm.nih.gov/pubmed/21339880
http://dx.doi.org/10.1364/BOE.2.000356

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038450/
https://www.ncbi.nlm.nih.gov/pubmed/21339880
http://dx.doi.org/10.1364/BOE.2.000356

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