Fast nonlinear spectral microscopy of in vivo human skin

An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at...

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Detalles Bibliográficos
Autores principales: Bader, Arjen N., Pena, Ana-Maria, Johan van Voskuilen, C., Palero, Jonathan A., Leroy, Frédéric, Colonna, Anne, Gerritsen, Hans C.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Dermatological Applications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038451/
https://www.ncbi.nlm.nih.gov/pubmed/21339881
http://dx.doi.org/10.1364/BOE.2.000365
Descripción
Sumario:An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.

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