Fast nonlinear spectral microscopy of in vivo human skin

An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at...

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Detalles Bibliográficos
Autores principales: Bader, Arjen N., Pena, Ana-Maria, Johan van Voskuilen, C., Palero, Jonathan A., Leroy, Frédéric, Colonna, Anne, Gerritsen, Hans C.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Dermatological Applications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038451/
https://www.ncbi.nlm.nih.gov/pubmed/21339881
http://dx.doi.org/10.1364/BOE.2.000365
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author Bader, Arjen N.
Pena, Ana-Maria
Johan van Voskuilen, C.
Palero, Jonathan A.
Leroy, Frédéric
Colonna, Anne
Gerritsen, Hans C.
author_facet Bader, Arjen N.
Pena, Ana-Maria
Johan van Voskuilen, C.
Palero, Jonathan A.
Leroy, Frédéric
Colonna, Anne
Gerritsen, Hans C.
author_sort Bader, Arjen N.
collection PubMed
description An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.
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spelling pubmed-30384512011-02-18 Fast nonlinear spectral microscopy of in vivo human skin Bader, Arjen N. Pena, Ana-Maria Johan van Voskuilen, C. Palero, Jonathan A. Leroy, Frédéric Colonna, Anne Gerritsen, Hans C. Biomed Opt Express Dermatological Applications An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed. Optical Society of America 2011-01-18 /pmc/articles/PMC3038451/ /pubmed/21339881 http://dx.doi.org/10.1364/BOE.2.000365 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Dermatological Applications
Bader, Arjen N.
Pena, Ana-Maria
Johan van Voskuilen, C.
Palero, Jonathan A.
Leroy, Frédéric
Colonna, Anne
Gerritsen, Hans C.
Fast nonlinear spectral microscopy of in vivo human skin
title Fast nonlinear spectral microscopy of in vivo human skin
title_full Fast nonlinear spectral microscopy of in vivo human skin
title_fullStr Fast nonlinear spectral microscopy of in vivo human skin
title_full_unstemmed Fast nonlinear spectral microscopy of in vivo human skin
title_short Fast nonlinear spectral microscopy of in vivo human skin
title_sort fast nonlinear spectral microscopy of in vivo human skin
topic Dermatological Applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038451/
https://www.ncbi.nlm.nih.gov/pubmed/21339881
http://dx.doi.org/10.1364/BOE.2.000365
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