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Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters
BACKGROUND: Chlamydomonas reinhardtii is a model system for the biology of unicellular green algae. Chemically regulated promoters, such as the nickel-inducible CYC6 or the low CO(2)-inducible CAH1 promoter, may prove useful for expressing, at precise times during its cell cycle, proteins with relev...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038898/ https://www.ncbi.nlm.nih.gov/pubmed/21266063 http://dx.doi.org/10.1186/1471-2229-11-22 |
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author | Ferrante, Paola Diener, Dennis R Rosenbaum, Joel L Giuliano, Giovanni |
author_facet | Ferrante, Paola Diener, Dennis R Rosenbaum, Joel L Giuliano, Giovanni |
author_sort | Ferrante, Paola |
collection | PubMed |
description | BACKGROUND: Chlamydomonas reinhardtii is a model system for the biology of unicellular green algae. Chemically regulated promoters, such as the nickel-inducible CYC6 or the low CO(2)-inducible CAH1 promoter, may prove useful for expressing, at precise times during its cell cycle, proteins with relevant biological functions, or complementing mutants in genes encoding such proteins. To this date, this has not been reported for the above promoters. RESULTS: We fused the CYC6 and CAH1 promoters to an HA-tagged RSP3 gene, encoding a protein of the flagellar radial spoke complex. The constructs were used for chemically regulated complementation of the pf14 mutant, carrying an ochre mutation in the RSP3 gene. 7 to 8% of the transformants showed cells with restored motility after induction with nickel or transfer to low CO(2 )conditions, but not in non-inducing conditions. Maximum complementation (5% motile cells) was reached with very different kinetics (5-6 hours for CAH1, 48 hours for CYC6). The two inducible promoters drive much lower levels of RSP3 protein expression than the constitutive PSAD promoter, which shows almost complete rescue of motility. CONCLUSIONS: To our knowledge, this is the first example of the use of the CYC6 or CAH1 promoters to perform a chemically regulated complementation of a Chlamydomonas mutant. Based on our data, the CYC6 and CAH1 promoters should be capable of fully complementing mutants in genes whose products exert their biological activity at low concentrations. |
format | Text |
id | pubmed-3038898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30388982011-02-15 Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters Ferrante, Paola Diener, Dennis R Rosenbaum, Joel L Giuliano, Giovanni BMC Plant Biol Research Article BACKGROUND: Chlamydomonas reinhardtii is a model system for the biology of unicellular green algae. Chemically regulated promoters, such as the nickel-inducible CYC6 or the low CO(2)-inducible CAH1 promoter, may prove useful for expressing, at precise times during its cell cycle, proteins with relevant biological functions, or complementing mutants in genes encoding such proteins. To this date, this has not been reported for the above promoters. RESULTS: We fused the CYC6 and CAH1 promoters to an HA-tagged RSP3 gene, encoding a protein of the flagellar radial spoke complex. The constructs were used for chemically regulated complementation of the pf14 mutant, carrying an ochre mutation in the RSP3 gene. 7 to 8% of the transformants showed cells with restored motility after induction with nickel or transfer to low CO(2 )conditions, but not in non-inducing conditions. Maximum complementation (5% motile cells) was reached with very different kinetics (5-6 hours for CAH1, 48 hours for CYC6). The two inducible promoters drive much lower levels of RSP3 protein expression than the constitutive PSAD promoter, which shows almost complete rescue of motility. CONCLUSIONS: To our knowledge, this is the first example of the use of the CYC6 or CAH1 promoters to perform a chemically regulated complementation of a Chlamydomonas mutant. Based on our data, the CYC6 and CAH1 promoters should be capable of fully complementing mutants in genes whose products exert their biological activity at low concentrations. BioMed Central 2011-01-25 /pmc/articles/PMC3038898/ /pubmed/21266063 http://dx.doi.org/10.1186/1471-2229-11-22 Text en Copyright ©2011 Ferrante et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ferrante, Paola Diener, Dennis R Rosenbaum, Joel L Giuliano, Giovanni Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title | Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title_full | Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title_fullStr | Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title_full_unstemmed | Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title_short | Nickel and low CO(2)-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
title_sort | nickel and low co(2)-controlled motility in chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038898/ https://www.ncbi.nlm.nih.gov/pubmed/21266063 http://dx.doi.org/10.1186/1471-2229-11-22 |
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