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Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038908/ https://www.ncbi.nlm.nih.gov/pubmed/21291552 http://dx.doi.org/10.1186/1742-4690-8-8 |
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author | Li, Bo Berry, Neil Ham, Claire Ferguson, Deborah Smith, Deborah Hall, Joanna Page, Mark Quartey-Papafio, Ruby Elsley, William Robinson, Mark Almond, Neil Stebbings, Richard |
author_facet | Li, Bo Berry, Neil Ham, Claire Ferguson, Deborah Smith, Deborah Hall, Joanna Page, Mark Quartey-Papafio, Ruby Elsley, William Robinson, Mark Almond, Neil Stebbings, Richard |
author_sort | Li, Bo |
collection | PubMed |
description | BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an early target of wild-type SIV infection and destruction, was determined at days 3, 7, 10, 21 and 125 post inoculation. RESULTS: In naive controls, modest frequencies of intestinal CD4+CCR5+ T cells were predominantly found within the LPL T(TrM-1 )and IEL T(TrM-2 )subsets. At day 3, LPL and IEL CD4+CCR5+ T(EM )cells were dramatically increased whilst less differentiated subsets were greatly reduced, consistent with activation-induced maturation. CCR5 expression remained high at day 7, although there was a shift in subset balance from CD4+CCR5+ T(EM )to less differentiated T(TrM-2 )cells. This increase in intestinal CD4+CCR5+ T cells preceded the peak of SIV RNA plasma loads measured at day 10. Greater than 65.9% depletion of intestinal CD4+CCR5+ T cells followed at day 10, but overall CD4+ T cell homeostasis was maintained by increased CD4+CCR5- T cells. At days 21 and 125, high numbers of intestinal CD4+CCR5- naive T(N )cells were detected concurrent with greatly increased CD4+CCR5+ LPL T(TrM-2 )and IEL T(EM )cells at day 125, yet SIV RNA plasma loads remained low. CONCLUSIONS: This increase in intestinal CD4+CCR5+ T cells, following vaccination with live attenuated SIV, does not correlate with target cell depletion as a mechanism of protection. Instead, increased intestinal CD4+CCR5+ T cells may correlate with or contribute to the protection conferred by vaccination with live attenuated SIV. |
format | Text |
id | pubmed-3038908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30389082011-02-15 Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells Li, Bo Berry, Neil Ham, Claire Ferguson, Deborah Smith, Deborah Hall, Joanna Page, Mark Quartey-Papafio, Ruby Elsley, William Robinson, Mark Almond, Neil Stebbings, Richard Retrovirology Research BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an early target of wild-type SIV infection and destruction, was determined at days 3, 7, 10, 21 and 125 post inoculation. RESULTS: In naive controls, modest frequencies of intestinal CD4+CCR5+ T cells were predominantly found within the LPL T(TrM-1 )and IEL T(TrM-2 )subsets. At day 3, LPL and IEL CD4+CCR5+ T(EM )cells were dramatically increased whilst less differentiated subsets were greatly reduced, consistent with activation-induced maturation. CCR5 expression remained high at day 7, although there was a shift in subset balance from CD4+CCR5+ T(EM )to less differentiated T(TrM-2 )cells. This increase in intestinal CD4+CCR5+ T cells preceded the peak of SIV RNA plasma loads measured at day 10. Greater than 65.9% depletion of intestinal CD4+CCR5+ T cells followed at day 10, but overall CD4+ T cell homeostasis was maintained by increased CD4+CCR5- T cells. At days 21 and 125, high numbers of intestinal CD4+CCR5- naive T(N )cells were detected concurrent with greatly increased CD4+CCR5+ LPL T(TrM-2 )and IEL T(EM )cells at day 125, yet SIV RNA plasma loads remained low. CONCLUSIONS: This increase in intestinal CD4+CCR5+ T cells, following vaccination with live attenuated SIV, does not correlate with target cell depletion as a mechanism of protection. Instead, increased intestinal CD4+CCR5+ T cells may correlate with or contribute to the protection conferred by vaccination with live attenuated SIV. BioMed Central 2011-02-03 /pmc/articles/PMC3038908/ /pubmed/21291552 http://dx.doi.org/10.1186/1742-4690-8-8 Text en Copyright ©2011 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Bo Berry, Neil Ham, Claire Ferguson, Deborah Smith, Deborah Hall, Joanna Page, Mark Quartey-Papafio, Ruby Elsley, William Robinson, Mark Almond, Neil Stebbings, Richard Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title | Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title_full | Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title_fullStr | Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title_full_unstemmed | Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title_short | Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells |
title_sort | vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal cd4+ccr5+ t cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038908/ https://www.ncbi.nlm.nih.gov/pubmed/21291552 http://dx.doi.org/10.1186/1742-4690-8-8 |
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