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Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells

BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an...

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Autores principales: Li, Bo, Berry, Neil, Ham, Claire, Ferguson, Deborah, Smith, Deborah, Hall, Joanna, Page, Mark, Quartey-Papafio, Ruby, Elsley, William, Robinson, Mark, Almond, Neil, Stebbings, Richard
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038908/
https://www.ncbi.nlm.nih.gov/pubmed/21291552
http://dx.doi.org/10.1186/1742-4690-8-8
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author Li, Bo
Berry, Neil
Ham, Claire
Ferguson, Deborah
Smith, Deborah
Hall, Joanna
Page, Mark
Quartey-Papafio, Ruby
Elsley, William
Robinson, Mark
Almond, Neil
Stebbings, Richard
author_facet Li, Bo
Berry, Neil
Ham, Claire
Ferguson, Deborah
Smith, Deborah
Hall, Joanna
Page, Mark
Quartey-Papafio, Ruby
Elsley, William
Robinson, Mark
Almond, Neil
Stebbings, Richard
author_sort Li, Bo
collection PubMed
description BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an early target of wild-type SIV infection and destruction, was determined at days 3, 7, 10, 21 and 125 post inoculation. RESULTS: In naive controls, modest frequencies of intestinal CD4+CCR5+ T cells were predominantly found within the LPL T(TrM-1 )and IEL T(TrM-2 )subsets. At day 3, LPL and IEL CD4+CCR5+ T(EM )cells were dramatically increased whilst less differentiated subsets were greatly reduced, consistent with activation-induced maturation. CCR5 expression remained high at day 7, although there was a shift in subset balance from CD4+CCR5+ T(EM )to less differentiated T(TrM-2 )cells. This increase in intestinal CD4+CCR5+ T cells preceded the peak of SIV RNA plasma loads measured at day 10. Greater than 65.9% depletion of intestinal CD4+CCR5+ T cells followed at day 10, but overall CD4+ T cell homeostasis was maintained by increased CD4+CCR5- T cells. At days 21 and 125, high numbers of intestinal CD4+CCR5- naive T(N )cells were detected concurrent with greatly increased CD4+CCR5+ LPL T(TrM-2 )and IEL T(EM )cells at day 125, yet SIV RNA plasma loads remained low. CONCLUSIONS: This increase in intestinal CD4+CCR5+ T cells, following vaccination with live attenuated SIV, does not correlate with target cell depletion as a mechanism of protection. Instead, increased intestinal CD4+CCR5+ T cells may correlate with or contribute to the protection conferred by vaccination with live attenuated SIV.
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spelling pubmed-30389082011-02-15 Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells Li, Bo Berry, Neil Ham, Claire Ferguson, Deborah Smith, Deborah Hall, Joanna Page, Mark Quartey-Papafio, Ruby Elsley, William Robinson, Mark Almond, Neil Stebbings, Richard Retrovirology Research BACKGROUND: Vaccination with live attenuated SIV can protect against detectable infection with wild-type virus. We have investigated whether target cell depletion contributes to the protection observed. Following vaccination with live attenuated SIV the frequency of intestinal CD4+CCR5+ T cells, an early target of wild-type SIV infection and destruction, was determined at days 3, 7, 10, 21 and 125 post inoculation. RESULTS: In naive controls, modest frequencies of intestinal CD4+CCR5+ T cells were predominantly found within the LPL T(TrM-1 )and IEL T(TrM-2 )subsets. At day 3, LPL and IEL CD4+CCR5+ T(EM )cells were dramatically increased whilst less differentiated subsets were greatly reduced, consistent with activation-induced maturation. CCR5 expression remained high at day 7, although there was a shift in subset balance from CD4+CCR5+ T(EM )to less differentiated T(TrM-2 )cells. This increase in intestinal CD4+CCR5+ T cells preceded the peak of SIV RNA plasma loads measured at day 10. Greater than 65.9% depletion of intestinal CD4+CCR5+ T cells followed at day 10, but overall CD4+ T cell homeostasis was maintained by increased CD4+CCR5- T cells. At days 21 and 125, high numbers of intestinal CD4+CCR5- naive T(N )cells were detected concurrent with greatly increased CD4+CCR5+ LPL T(TrM-2 )and IEL T(EM )cells at day 125, yet SIV RNA plasma loads remained low. CONCLUSIONS: This increase in intestinal CD4+CCR5+ T cells, following vaccination with live attenuated SIV, does not correlate with target cell depletion as a mechanism of protection. Instead, increased intestinal CD4+CCR5+ T cells may correlate with or contribute to the protection conferred by vaccination with live attenuated SIV. BioMed Central 2011-02-03 /pmc/articles/PMC3038908/ /pubmed/21291552 http://dx.doi.org/10.1186/1742-4690-8-8 Text en Copyright ©2011 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Li, Bo
Berry, Neil
Ham, Claire
Ferguson, Deborah
Smith, Deborah
Hall, Joanna
Page, Mark
Quartey-Papafio, Ruby
Elsley, William
Robinson, Mark
Almond, Neil
Stebbings, Richard
Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title_full Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title_fullStr Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title_full_unstemmed Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title_short Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
title_sort vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal cd4+ccr5+ t cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038908/
https://www.ncbi.nlm.nih.gov/pubmed/21291552
http://dx.doi.org/10.1186/1742-4690-8-8
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