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Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)

Although many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampa...

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Autores principales: Perasso, Luisa, Cogo, Carla Emilia, Giunti, Debora, Gandolfo, Carlo, Ruggeri, Piero, Uccelli, Antonio, Balestrino, Maurizio
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039275/
https://www.ncbi.nlm.nih.gov/pubmed/21331297
http://dx.doi.org/10.1155/2010/534925
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author Perasso, Luisa
Cogo, Carla Emilia
Giunti, Debora
Gandolfo, Carlo
Ruggeri, Piero
Uccelli, Antonio
Balestrino, Maurizio
author_facet Perasso, Luisa
Cogo, Carla Emilia
Giunti, Debora
Gandolfo, Carlo
Ruggeri, Piero
Uccelli, Antonio
Balestrino, Maurizio
author_sort Perasso, Luisa
collection PubMed
description Although many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampal CA1 region. We tested the hypothesis that intravenous infusion of bone marrowderived stromal cells (mesenchimal stem cells, MSC) reduce brain damage after transient global ischemia. In adult male Sprague-Dawley rats transient global ischemia was induced using bilateral common carotid artery occlusion for 20 min in addition to controlled hypotension. Five days after, the animals were anaesthetized with urethane and the brain was fixed, sectioned and stained with hematoxylin-eosin to investigate histological damage. MSC did not fully protect against ischemic damage, as the number of viable neurons in this group was lower than in normal (sham-operated) rats. However, in MSC-treated rats the number of viable CA1 pyramidal neurons was significally higher than in rats that had been subjected to ischemia but not treated with MSC. We conclude that intravenous administration of MSC after transient global ischemia reduces hippocampal damage.
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spelling pubmed-30392752011-02-17 Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO) Perasso, Luisa Cogo, Carla Emilia Giunti, Debora Gandolfo, Carlo Ruggeri, Piero Uccelli, Antonio Balestrino, Maurizio Neural Plast Research Article Although many studies have shown that administration of stem cells after focal cerebral ischemia improves brain damage, very little data are available concerning the damage induced by global cerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including the hippocampal CA1 region. We tested the hypothesis that intravenous infusion of bone marrowderived stromal cells (mesenchimal stem cells, MSC) reduce brain damage after transient global ischemia. In adult male Sprague-Dawley rats transient global ischemia was induced using bilateral common carotid artery occlusion for 20 min in addition to controlled hypotension. Five days after, the animals were anaesthetized with urethane and the brain was fixed, sectioned and stained with hematoxylin-eosin to investigate histological damage. MSC did not fully protect against ischemic damage, as the number of viable neurons in this group was lower than in normal (sham-operated) rats. However, in MSC-treated rats the number of viable CA1 pyramidal neurons was significally higher than in rats that had been subjected to ischemia but not treated with MSC. We conclude that intravenous administration of MSC after transient global ischemia reduces hippocampal damage. Hindawi Publishing Corporation 2010 2010-12-19 /pmc/articles/PMC3039275/ /pubmed/21331297 http://dx.doi.org/10.1155/2010/534925 Text en Copyright © 2010 Luisa Perasso et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Perasso, Luisa
Cogo, Carla Emilia
Giunti, Debora
Gandolfo, Carlo
Ruggeri, Piero
Uccelli, Antonio
Balestrino, Maurizio
Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title_full Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title_fullStr Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title_full_unstemmed Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title_short Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)
title_sort systemic administration of mesenchymal stem cells increases neuron survival after global cerebral ischemia in vivo (2vo)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039275/
https://www.ncbi.nlm.nih.gov/pubmed/21331297
http://dx.doi.org/10.1155/2010/534925
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