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Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition

The effects of amino acids addition on cell growth, glutathione biosynthesis, glutathione distribution, and the intracellular oxidation-reduction environment of Candida utilis SZU 07-01 during selenium enrichment were investigated in this study. Most amino acids under appropriate concentrations have...

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Autores principales: Xiaoguang, Ge, Dahui, Wang, Gongyuan, Wei, Min, Nie, Na, Shao
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039456/
https://www.ncbi.nlm.nih.gov/pubmed/21350658
http://dx.doi.org/10.4061/2011/238456
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author Xiaoguang, Ge
Dahui, Wang
Gongyuan, Wei
Min, Nie
Na, Shao
author_facet Xiaoguang, Ge
Dahui, Wang
Gongyuan, Wei
Min, Nie
Na, Shao
author_sort Xiaoguang, Ge
collection PubMed
description The effects of amino acids addition on cell growth, glutathione biosynthesis, glutathione distribution, and the intracellular oxidation-reduction environment of Candida utilis SZU 07-01 during selenium enrichment were investigated in this study. Most amino acids under appropriate concentrations have positive effects on cell growth of the yeast strain, except for phenylalanine and proline, compared with the control without amino acid addition. The bioconversion of selenite to organic selenium induced the reduction of glutathione synthesis and intracellular distribution of glutathione. However, amino acids including cysteine, glutamine, glutamic acid, isoleucine, leucine, and tyrosine could effectively promote the selenium-enriched yeast to elevate glutathione production, especially increasing the intracellular glutathione content. Moreover, addition of these six different amino acids apparently decreased malondialdehyde concentration and recovered the normal intracellular redox environment of the selenium-enriched C. utilis SZU 07-01. The improvement of physiological characteristic of the selenium-enriched yeast by increasing intracellular glutathione content and lowering malondialdehyde content will undoubtedly help to widen application of selenium-enriched yeast as food or feed additives.
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spelling pubmed-30394562011-02-24 Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition Xiaoguang, Ge Dahui, Wang Gongyuan, Wei Min, Nie Na, Shao Biotechnol Res Int Research Article The effects of amino acids addition on cell growth, glutathione biosynthesis, glutathione distribution, and the intracellular oxidation-reduction environment of Candida utilis SZU 07-01 during selenium enrichment were investigated in this study. Most amino acids under appropriate concentrations have positive effects on cell growth of the yeast strain, except for phenylalanine and proline, compared with the control without amino acid addition. The bioconversion of selenite to organic selenium induced the reduction of glutathione synthesis and intracellular distribution of glutathione. However, amino acids including cysteine, glutamine, glutamic acid, isoleucine, leucine, and tyrosine could effectively promote the selenium-enriched yeast to elevate glutathione production, especially increasing the intracellular glutathione content. Moreover, addition of these six different amino acids apparently decreased malondialdehyde concentration and recovered the normal intracellular redox environment of the selenium-enriched C. utilis SZU 07-01. The improvement of physiological characteristic of the selenium-enriched yeast by increasing intracellular glutathione content and lowering malondialdehyde content will undoubtedly help to widen application of selenium-enriched yeast as food or feed additives. SAGE-Hindawi Access to Research 2010-09-19 /pmc/articles/PMC3039456/ /pubmed/21350658 http://dx.doi.org/10.4061/2011/238456 Text en Copyright © 2011 Ge Xiaoguang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xiaoguang, Ge
Dahui, Wang
Gongyuan, Wei
Min, Nie
Na, Shao
Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title_full Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title_fullStr Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title_full_unstemmed Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title_short Improvement of Physiological Characteristic of Selenium-Enriched Candida utilis with Amino Acids Addition
title_sort improvement of physiological characteristic of selenium-enriched candida utilis with amino acids addition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039456/
https://www.ncbi.nlm.nih.gov/pubmed/21350658
http://dx.doi.org/10.4061/2011/238456
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