Cargando…

Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis

BACKGROUND: Sessile bivalves of the genus Mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. Mortality events caused by infective agents and parasites apparently occur le...

Descripción completa

Detalles Bibliográficos
Autores principales: Venier, Paola, Varotto, Laura, Rosani, Umberto, Millino, Caterina, Celegato, Barbara, Bernante, Filippo, Lanfranchi, Gerolamo, Novoa, Beatriz, Roch, Philippe, Figueras, Antonio, Pallavicini, Alberto
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039611/
https://www.ncbi.nlm.nih.gov/pubmed/21269501
http://dx.doi.org/10.1186/1471-2164-12-69
_version_ 1782198206379065344
author Venier, Paola
Varotto, Laura
Rosani, Umberto
Millino, Caterina
Celegato, Barbara
Bernante, Filippo
Lanfranchi, Gerolamo
Novoa, Beatriz
Roch, Philippe
Figueras, Antonio
Pallavicini, Alberto
author_facet Venier, Paola
Varotto, Laura
Rosani, Umberto
Millino, Caterina
Celegato, Barbara
Bernante, Filippo
Lanfranchi, Gerolamo
Novoa, Beatriz
Roch, Philippe
Figueras, Antonio
Pallavicini, Alberto
author_sort Venier, Paola
collection PubMed
description BACKGROUND: Sessile bivalves of the genus Mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. Mortality events caused by infective agents and parasites apparently occur less in mussels than in other bivalves but the molecular basis of such evidence is unknown. The arrangement of Mytibase, interactive catalogue of 7,112 transcripts of M. galloprovincialis, offered us the opportunity to look for gene sequences relevant to the host defences, in particular the innate immunity related genes. RESULTS: We have explored and described the Mytibase sequence clusters and singletons having a putative role in recognition, intracellular signalling, and neutralization of potential pathogens in M. galloprovincialis. Automatically assisted searches of protein signatures and manually cured sequence analysis confirmed the molecular diversity of recognition/effector molecules such as the antimicrobial peptides and many carbohydrate binding proteins. Molecular motifs identifying complement C1q, C-type lectins and fibrinogen-like transcripts emerged as the most abundant in the Mytibase collection whereas, conversely, sequence motifs denoting the regulatory cytokine MIF and cytokine-related transcripts represent singular and unexpected findings. Using a cross-search strategy, 1,820 putatively immune-related sequences were selected to design oligonucleotide probes and define a species-specific Immunochip (DNA microarray). The Immunochip performance was tested with hemolymph RNAs from mussels injected with Vibrio splendidus at 3 and 48 hours post-treatment. A total of 143 and 262 differentially expressed genes exemplify the early and late hemocyte response of the Vibrio-challenged mussels, respectively, with AMP trends confirmed by qPCR and clear modulation of interrelated signalling pathways. CONCLUSIONS: The Mytibase collection is rich in gene transcripts modulated in response to antigenic stimuli and represents an interesting window for looking at the mussel immunome (transcriptomes mediating the mussel response to non-self or abnormal antigens). On this basis, we have defined a new microarray platform, a mussel Immunochip, as a flexible tool for the experimental validation of immune-candidate sequences, and tested its performance on Vibrio-activated mussel hemocytes. The microarray platform and related expression data can be regarded as a step forward in the study of the adaptive response of the Mytilus species to an evolving microbial world.
format Text
id pubmed-3039611
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30396112011-02-16 Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis Venier, Paola Varotto, Laura Rosani, Umberto Millino, Caterina Celegato, Barbara Bernante, Filippo Lanfranchi, Gerolamo Novoa, Beatriz Roch, Philippe Figueras, Antonio Pallavicini, Alberto BMC Genomics Research Article BACKGROUND: Sessile bivalves of the genus Mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. Mortality events caused by infective agents and parasites apparently occur less in mussels than in other bivalves but the molecular basis of such evidence is unknown. The arrangement of Mytibase, interactive catalogue of 7,112 transcripts of M. galloprovincialis, offered us the opportunity to look for gene sequences relevant to the host defences, in particular the innate immunity related genes. RESULTS: We have explored and described the Mytibase sequence clusters and singletons having a putative role in recognition, intracellular signalling, and neutralization of potential pathogens in M. galloprovincialis. Automatically assisted searches of protein signatures and manually cured sequence analysis confirmed the molecular diversity of recognition/effector molecules such as the antimicrobial peptides and many carbohydrate binding proteins. Molecular motifs identifying complement C1q, C-type lectins and fibrinogen-like transcripts emerged as the most abundant in the Mytibase collection whereas, conversely, sequence motifs denoting the regulatory cytokine MIF and cytokine-related transcripts represent singular and unexpected findings. Using a cross-search strategy, 1,820 putatively immune-related sequences were selected to design oligonucleotide probes and define a species-specific Immunochip (DNA microarray). The Immunochip performance was tested with hemolymph RNAs from mussels injected with Vibrio splendidus at 3 and 48 hours post-treatment. A total of 143 and 262 differentially expressed genes exemplify the early and late hemocyte response of the Vibrio-challenged mussels, respectively, with AMP trends confirmed by qPCR and clear modulation of interrelated signalling pathways. CONCLUSIONS: The Mytibase collection is rich in gene transcripts modulated in response to antigenic stimuli and represents an interesting window for looking at the mussel immunome (transcriptomes mediating the mussel response to non-self or abnormal antigens). On this basis, we have defined a new microarray platform, a mussel Immunochip, as a flexible tool for the experimental validation of immune-candidate sequences, and tested its performance on Vibrio-activated mussel hemocytes. The microarray platform and related expression data can be regarded as a step forward in the study of the adaptive response of the Mytilus species to an evolving microbial world. BioMed Central 2011-01-26 /pmc/articles/PMC3039611/ /pubmed/21269501 http://dx.doi.org/10.1186/1471-2164-12-69 Text en Copyright ©2011 Venier et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Venier, Paola
Varotto, Laura
Rosani, Umberto
Millino, Caterina
Celegato, Barbara
Bernante, Filippo
Lanfranchi, Gerolamo
Novoa, Beatriz
Roch, Philippe
Figueras, Antonio
Pallavicini, Alberto
Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title_full Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title_fullStr Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title_full_unstemmed Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title_short Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis
title_sort insights into the innate immunity of the mediterranean mussel mytilus galloprovincialis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039611/
https://www.ncbi.nlm.nih.gov/pubmed/21269501
http://dx.doi.org/10.1186/1471-2164-12-69
work_keys_str_mv AT venierpaola insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT varottolaura insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT rosaniumberto insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT millinocaterina insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT celegatobarbara insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT bernantefilippo insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT lanfranchigerolamo insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT novoabeatriz insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT rochphilippe insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT figuerasantonio insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis
AT pallavicinialberto insightsintotheinnateimmunityofthemediterraneanmusselmytilusgalloprovincialis