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Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B(1) (AFB(1)) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibod...

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Detalles Bibliográficos
Autores principales:	Zhao, Jian-Xin, Yang, Lian, Gu, Zhen-Nan, Chen, Hai-Qin, Tian, Feng-Wei, Chen, Yong-Quan, Zhang, Hao, Chen, Wei
Formato:	Texto
Lenguaje:	English
Publicado:	Molecular Diversity Preservation International (MDPI) 2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039938/ https://www.ncbi.nlm.nih.gov/pubmed/21339972 http://dx.doi.org/10.3390/ijms12010001

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