Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells

In this study, Leydig cells were purified from 70 day-old Sprague Dawley male rats and incubated with 10 and 100 μg/mL of methanol extract of Basella alba (MEBa) for 4 hours followed by the evaluation of cell viability, steroid (testosterone and estradiol) production, and the level of aromatase mRNA...

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Detalles Bibliográficos
Autores principales: Nantia, Edouard Akono, Travert, Carine, Manfo, Faustin-Pascal T., Carreau, Serge, Monsees, Thomas K., Moundipa, Paul Fewou
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039958/
https://www.ncbi.nlm.nih.gov/pubmed/21339992
http://dx.doi.org/10.3390/ijms12010376
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author Nantia, Edouard Akono
Travert, Carine
Manfo, Faustin-Pascal T.
Carreau, Serge
Monsees, Thomas K.
Moundipa, Paul Fewou
author_facet Nantia, Edouard Akono
Travert, Carine
Manfo, Faustin-Pascal T.
Carreau, Serge
Monsees, Thomas K.
Moundipa, Paul Fewou
author_sort Nantia, Edouard Akono
collection PubMed
description In this study, Leydig cells were purified from 70 day-old Sprague Dawley male rats and incubated with 10 and 100 μg/mL of methanol extract of Basella alba (MEBa) for 4 hours followed by the evaluation of cell viability, steroid (testosterone and estradiol) production, and the level of aromatase mRNA. Results showed that MEBa did not affect Leydig cell viability. At the concentration of 10 μg/mL, MEBa significantly stimulated testosterone and estradiol production (p < 0.01 and p < 0.03, respectively), and enhanced aromatase mRNA level (p < 0.04). These observations suggest that MEBa directly stimulated testosterone, estradiol and aromatase mRNA levels in isolated Leydig cells.
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spelling pubmed-30399582011-02-18 Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells Nantia, Edouard Akono Travert, Carine Manfo, Faustin-Pascal T. Carreau, Serge Monsees, Thomas K. Moundipa, Paul Fewou Int J Mol Sci Article In this study, Leydig cells were purified from 70 day-old Sprague Dawley male rats and incubated with 10 and 100 μg/mL of methanol extract of Basella alba (MEBa) for 4 hours followed by the evaluation of cell viability, steroid (testosterone and estradiol) production, and the level of aromatase mRNA. Results showed that MEBa did not affect Leydig cell viability. At the concentration of 10 μg/mL, MEBa significantly stimulated testosterone and estradiol production (p < 0.01 and p < 0.03, respectively), and enhanced aromatase mRNA level (p < 0.04). These observations suggest that MEBa directly stimulated testosterone, estradiol and aromatase mRNA levels in isolated Leydig cells. Molecular Diversity Preservation International (MDPI) 2011-01-14 /pmc/articles/PMC3039958/ /pubmed/21339992 http://dx.doi.org/10.3390/ijms12010376 Text en © 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Nantia, Edouard Akono
Travert, Carine
Manfo, Faustin-Pascal T.
Carreau, Serge
Monsees, Thomas K.
Moundipa, Paul Fewou
Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title_full Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title_fullStr Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title_full_unstemmed Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title_short Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells
title_sort effects of the methanol extract of basella alba l (basellaceae) on steroid production in leydig cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039958/
https://www.ncbi.nlm.nih.gov/pubmed/21339992
http://dx.doi.org/10.3390/ijms12010376
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