Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Da-Wei, Li, Yu, Li, Zhou-Qi
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039984/
https://www.ncbi.nlm.nih.gov/pubmed/21340018
http://dx.doi.org/10.3390/ijms12010857
_version_ 1782198260822179840
author Wang, Da-Wei
Li, Yu
Li, Zhou-Qi
author_facet Wang, Da-Wei
Li, Yu
Li, Zhou-Qi
author_sort Wang, Da-Wei
collection PubMed
description Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64 AFLP primer combinations screened, primer combination E-ACA/M-CTT produced a 350 bp male-specific marker. This 350 bp AFLP marker was converted into a 247 bp Sequence Characterized Amplified Region (SCAR) marker. Results suggest that the SCAR marker can be utilized for early sexual identification in Eucommia ulmoides Oliv., and it will greatly facilitate future breeding programs.
format Text
id pubmed-3039984
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-30399842011-02-18 Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv. Wang, Da-Wei Li, Yu Li, Zhou-Qi Int J Mol Sci Article Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64 AFLP primer combinations screened, primer combination E-ACA/M-CTT produced a 350 bp male-specific marker. This 350 bp AFLP marker was converted into a 247 bp Sequence Characterized Amplified Region (SCAR) marker. Results suggest that the SCAR marker can be utilized for early sexual identification in Eucommia ulmoides Oliv., and it will greatly facilitate future breeding programs. Molecular Diversity Preservation International (MDPI) 2011-01-24 /pmc/articles/PMC3039984/ /pubmed/21340018 http://dx.doi.org/10.3390/ijms12010857 Text en © 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Da-Wei
Li, Yu
Li, Zhou-Qi
Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title_full Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title_fullStr Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title_full_unstemmed Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title_short Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.
title_sort identification of a male-specific amplified fragment length polymorphism (aflp) and a sequence characterized amplified region (scar) marker in eucommia ulmoides oliv.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039984/
https://www.ncbi.nlm.nih.gov/pubmed/21340018
http://dx.doi.org/10.3390/ijms12010857
work_keys_str_mv AT wangdawei identificationofamalespecificamplifiedfragmentlengthpolymorphismaflpandasequencecharacterizedamplifiedregionscarmarkerineucommiaulmoidesoliv
AT liyu identificationofamalespecificamplifiedfragmentlengthpolymorphismaflpandasequencecharacterizedamplifiedregionscarmarkerineucommiaulmoidesoliv
AT lizhouqi identificationofamalespecificamplifiedfragmentlengthpolymorphismaflpandasequencecharacterizedamplifiedregionscarmarkerineucommiaulmoidesoliv

Ejemplares similares