Cargando…

Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser

BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa infection represents a main cause of morbidity and mortality among immunocompromised patients. This study describes a fatal epidemic of P. aeruginosa that occurred in a hematology unit in Italy. METHODS: Retrospective cohort study, prospective surveil...

Descripción completa

Detalles Bibliográficos
Autores principales: Lanini, Simone, D'Arezzo, Silvia, Puro, Vincenzo, Martini, Lorena, Imperi, Francesco, Piselli, Pierluca, Montanaro, Marco, Paoletti, Simonetta, Visca, Paolo, Ippolito, Giuseppe
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040201/
https://www.ncbi.nlm.nih.gov/pubmed/21359222
http://dx.doi.org/10.1371/journal.pone.0017064
_version_ 1782198290321768448
author Lanini, Simone
D'Arezzo, Silvia
Puro, Vincenzo
Martini, Lorena
Imperi, Francesco
Piselli, Pierluca
Montanaro, Marco
Paoletti, Simonetta
Visca, Paolo
Ippolito, Giuseppe
author_facet Lanini, Simone
D'Arezzo, Silvia
Puro, Vincenzo
Martini, Lorena
Imperi, Francesco
Piselli, Pierluca
Montanaro, Marco
Paoletti, Simonetta
Visca, Paolo
Ippolito, Giuseppe
author_sort Lanini, Simone
collection PubMed
description BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa infection represents a main cause of morbidity and mortality among immunocompromised patients. This study describes a fatal epidemic of P. aeruginosa that occurred in a hematology unit in Italy. METHODS: Retrospective cohort study, prospective surveillance, auditing, extensive testing on healthcare workers and environmental investigation were performed to define the dynamics and potential causes of transmission. RAPD, macrorestriction analyses and sequence typing were used to define relationships between P. aeruginosa isolates. RESULTS: Eighteen cases of infection were identified in the different phases of the investigation. Of these, five constitute a significant molecular cluster of infection. A P. aeruginosa strain with the same genetic fingerprint and sequence type (ST175) as clinical isolates strain was also isolated from a heavily contaminated triclosan soap dispenser. DISCUSSION AND CONCLUSIONS: Our results are consistent with the hypothesis that patients became indirectly infected, e.g., during central venous catheter handling through contaminated items, and that the triclosan soap dispenser acted as a common continuous source of P. aeruginosa infection. Since P. aeruginosa is intrinsically unsusceptible to triclosan, the use of triclosan-based disinfectant formulations should be avoided in those healthcare settings hosting patients at high risk of P. aeruginosa infection.
format Text
id pubmed-3040201
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30402012011-02-25 Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser Lanini, Simone D'Arezzo, Silvia Puro, Vincenzo Martini, Lorena Imperi, Francesco Piselli, Pierluca Montanaro, Marco Paoletti, Simonetta Visca, Paolo Ippolito, Giuseppe PLoS One Research Article BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa infection represents a main cause of morbidity and mortality among immunocompromised patients. This study describes a fatal epidemic of P. aeruginosa that occurred in a hematology unit in Italy. METHODS: Retrospective cohort study, prospective surveillance, auditing, extensive testing on healthcare workers and environmental investigation were performed to define the dynamics and potential causes of transmission. RAPD, macrorestriction analyses and sequence typing were used to define relationships between P. aeruginosa isolates. RESULTS: Eighteen cases of infection were identified in the different phases of the investigation. Of these, five constitute a significant molecular cluster of infection. A P. aeruginosa strain with the same genetic fingerprint and sequence type (ST175) as clinical isolates strain was also isolated from a heavily contaminated triclosan soap dispenser. DISCUSSION AND CONCLUSIONS: Our results are consistent with the hypothesis that patients became indirectly infected, e.g., during central venous catheter handling through contaminated items, and that the triclosan soap dispenser acted as a common continuous source of P. aeruginosa infection. Since P. aeruginosa is intrinsically unsusceptible to triclosan, the use of triclosan-based disinfectant formulations should be avoided in those healthcare settings hosting patients at high risk of P. aeruginosa infection. Public Library of Science 2011-02-16 /pmc/articles/PMC3040201/ /pubmed/21359222 http://dx.doi.org/10.1371/journal.pone.0017064 Text en Lanini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lanini, Simone
D'Arezzo, Silvia
Puro, Vincenzo
Martini, Lorena
Imperi, Francesco
Piselli, Pierluca
Montanaro, Marco
Paoletti, Simonetta
Visca, Paolo
Ippolito, Giuseppe
Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title_full Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title_fullStr Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title_full_unstemmed Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title_short Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser
title_sort molecular epidemiology of a pseudomonas aeruginosa hospital outbreak driven by a contaminated disinfectant-soap dispenser
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040201/
https://www.ncbi.nlm.nih.gov/pubmed/21359222
http://dx.doi.org/10.1371/journal.pone.0017064
work_keys_str_mv AT laninisimone molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT darezzosilvia molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT purovincenzo molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT martinilorena molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT imperifrancesco molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT pisellipierluca molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT montanaromarco molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT paolettisimonetta molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT viscapaolo molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser
AT ippolitogiuseppe molecularepidemiologyofapseudomonasaeruginosahospitaloutbreakdrivenbyacontaminateddisinfectantsoapdispenser