A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica

A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content...

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Detalles Bibliográficos
Autores principales: Unnikrishnan, K. P., Raja, S. Sudhakar, Balachandran, Indira
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2008
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040894/
https://www.ncbi.nlm.nih.gov/pubmed/21369461
http://dx.doi.org/10.4103/0250-474X.49142
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author Unnikrishnan, K. P.
Raja, S. Sudhakar
Balachandran, Indira
author_facet Unnikrishnan, K. P.
Raja, S. Sudhakar
Balachandran, Indira
author_sort Unnikrishnan, K. P.
collection PubMed
description A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.
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spelling pubmed-30408942011-03-02 A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica Unnikrishnan, K. P. Raja, S. Sudhakar Balachandran, Indira Indian J Pharm Sci Short Communication A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive. Medknow Publications 2008 /pmc/articles/PMC3040894/ /pubmed/21369461 http://dx.doi.org/10.4103/0250-474X.49142 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Unnikrishnan, K. P.
Raja, S. Sudhakar
Balachandran, Indira
A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title_full A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title_fullStr A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title_full_unstemmed A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title_short A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica
title_sort reverse phase hplc-uv and hptlc methods for determination of plumbagin in plumbago indica and plumbago zeylanica
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040894/
https://www.ncbi.nlm.nih.gov/pubmed/21369461
http://dx.doi.org/10.4103/0250-474X.49142
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