Cargando…

Mapping of origin of replication in Themococcales

Genome replication is a crucial and essential process for the continuity of life.In all organisms it starts at a specific region of the genome known as origin of replication (Ori) site. The number of Ori sites varies in prokaryotes and eukaryotes. Replication starts at a single Ori site in bacteria,...

Descripción completa

Detalles Bibliográficos
Autores principales: Ojha, Krishna K, Swati, D
Formato: Texto
Lenguaje:English
Publicado: Biomedical Informatics 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041001/
https://www.ncbi.nlm.nih.gov/pubmed/21364800
_version_ 1782198399522570240
author Ojha, Krishna K
Swati, D
author_facet Ojha, Krishna K
Swati, D
author_sort Ojha, Krishna K
collection PubMed
description Genome replication is a crucial and essential process for the continuity of life.In all organisms it starts at a specific region of the genome known as origin of replication (Ori) site. The number of Ori sites varies in prokaryotes and eukaryotes. Replication starts at a single Ori site in bacteria, but in eukaryotes multiple Ori sites are used for fast copying across all chromosomes. The situation becomes complex in archaea, where some groups have single and others have multiple origins of replication. Themococcales, are a hyperthermophilic order of archaea. They are anaerobes and heterotrophs-peptide fermenters, sulphate reducers, methanogens being some of the examples of metabolic types. In this paper we have applied a combination of multiple in silico approaches - Z curve, the cell division cycle (cdc6) gene location and location of consensus origin recognition box (ORB) sequences for location of origin of replication in Thermococcus onnurineus, Thermococcus gammatolerans and other Themococcales and compared the results to that of the well-documented case of Pyrococcus abyssi. The motivation behind this study is to find the number of Ori sites based on the data available for members of this order. Results from this in silico analysis show that the Themococcales have a single origin of replication.
format Text
id pubmed-3041001
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Biomedical Informatics
record_format MEDLINE/PubMed
spelling pubmed-30410012011-03-01 Mapping of origin of replication in Themococcales Ojha, Krishna K Swati, D Bioinformation Hypothesis Genome replication is a crucial and essential process for the continuity of life.In all organisms it starts at a specific region of the genome known as origin of replication (Ori) site. The number of Ori sites varies in prokaryotes and eukaryotes. Replication starts at a single Ori site in bacteria, but in eukaryotes multiple Ori sites are used for fast copying across all chromosomes. The situation becomes complex in archaea, where some groups have single and others have multiple origins of replication. Themococcales, are a hyperthermophilic order of archaea. They are anaerobes and heterotrophs-peptide fermenters, sulphate reducers, methanogens being some of the examples of metabolic types. In this paper we have applied a combination of multiple in silico approaches - Z curve, the cell division cycle (cdc6) gene location and location of consensus origin recognition box (ORB) sequences for location of origin of replication in Thermococcus onnurineus, Thermococcus gammatolerans and other Themococcales and compared the results to that of the well-documented case of Pyrococcus abyssi. The motivation behind this study is to find the number of Ori sites based on the data available for members of this order. Results from this in silico analysis show that the Themococcales have a single origin of replication. Biomedical Informatics 2010-11-01 /pmc/articles/PMC3041001/ /pubmed/21364800 Text en © 2010 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Hypothesis
Ojha, Krishna K
Swati, D
Mapping of origin of replication in Themococcales
title Mapping of origin of replication in Themococcales
title_full Mapping of origin of replication in Themococcales
title_fullStr Mapping of origin of replication in Themococcales
title_full_unstemmed Mapping of origin of replication in Themococcales
title_short Mapping of origin of replication in Themococcales
title_sort mapping of origin of replication in themococcales
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041001/
https://www.ncbi.nlm.nih.gov/pubmed/21364800
work_keys_str_mv AT ojhakrishnak mappingoforiginofreplicationinthemococcales
AT swatid mappingoforiginofreplicationinthemococcales