Phenotype Sequencing: Identifying the Genes That Cause a Phenotype Directly from Pooled Sequencing of Independent Mutants

Random mutagenesis and phenotype screening provide a powerful method for dissecting microbial functions, but their results can be laborious to analyze experimentally. Each mutant strain may contain 50–100 random mutations, necessitating extensive functional experiments to determine which one causes...

Descripción completa

Detalles Bibliográficos
Autores principales: Harper, Marc A., Chen, Zugen, Toy, Traci, Machado, Iara M. P., Nelson, Stanley F., Liao, James C., Lee, Christopher J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041756/
https://www.ncbi.nlm.nih.gov/pubmed/21364744
http://dx.doi.org/10.1371/journal.pone.0016517

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041756/
https://www.ncbi.nlm.nih.gov/pubmed/21364744
http://dx.doi.org/10.1371/journal.pone.0016517

Ejemplares similares