Cargando…

Phenotype Sequencing: Identifying the Genes That Cause a Phenotype Directly from Pooled Sequencing of Independent Mutants

Random mutagenesis and phenotype screening provide a powerful method for dissecting microbial functions, but their results can be laborious to analyze experimentally. Each mutant strain may contain 50–100 random mutations, necessitating extensive functional experiments to determine which one causes...

Descripción completa

Detalles Bibliográficos
Autores principales:	Harper, Marc A., Chen, Zugen, Toy, Traci, Machado, Iara M. P., Nelson, Stanley F., Liao, James C., Lee, Christopher J.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041756/ https://www.ncbi.nlm.nih.gov/pubmed/21364744 http://dx.doi.org/10.1371/journal.pone.0016517

Ejemplares similares

Comprehensive Detection of Genes Causing a Phenotype Using Phenotype Sequencing and Pathway Analysis
por: Harper, Marc, et al.
Publicado: (2014)

Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants
por: Bronner, Iraad F., et al.
Publicado: (2016)

Identifying the genetic causes of phenotypically diagnosed Pakistani mucopolysaccharidoses patients by whole genome sequencing
por: Gul, Rutaba, et al.
Publicado: (2023)

Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing
por: Vandewalle, Kristof, et al.
Publicado: (2015)

Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype
por: Lichius, Alexander, et al.
Publicado: (2015)

Identification of allele-specific alternative mRNA processing via transcriptome sequencing
por: Li, Gang, et al.
Publicado: (2012)

Abstract 11: Osteoporotic Phenotype of Newly Identified Zebrafish Mutant
por: Bidny, Sophia A., et al.
Publicado: (2018)

Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host‐Microbe Interactions
por: Uhse, Simon, et al.
Publicado: (2019)

Erratum to: Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype
por: Lichius, Alexander, et al.
Publicado: (2015)

From phenotypical investigation to RNA-sequencing for gene expression analysis: A workflow for single and pooled rare cells
por: Rossi, Tania, et al.
Publicado: (2022)

Identification of loci controlling timing of stem elongation in red clover using genotyping by sequencing of pooled phenotypic extremes
por: Ergon, Åshild, et al.
Publicado: (2022)

Identifying genetic determinants of complex phenotypes from whole genome sequence data
por: Long, George S., et al.
Publicado: (2019)

Identifying phenotype-associated subpopulations by integrating bulk and single-cell sequencing data
por: Sun, Duanchen, et al.
Publicado: (2022)

Molecular diagnosis of putative Stargardt disease probands by exome sequencing
por: Strom, Samuel P, et al.
Publicado: (2012)

RNA Sequencing of Pooled Samples Effectively Identifies Differentially Expressed Genes
por: Ko, Bokang, et al.
Publicado: (2023)

Testing a candidate meiotic drive locus identified by pool sequencing
por: Barbash, Daniel A, et al.
Publicado: (2023)

U87MG Decoded: The Genomic Sequence of a Cytogenetically Aberrant Human Cancer Cell Line
por: Clark, Michael James, et al.
Publicado: (2010)

Next-generation sequencing identifies unexpected genotype-phenotype correlations in patients with retinitis pigmentosa
por: Birtel, Johannes, et al.
Publicado: (2018)

Combining Whole-Genome Sequencing and Multimodel Phenotyping To Identify Genetic Predictors of Salmonella Virulence
por: Crouse, Alanna, et al.
Publicado: (2020)

Rapid gene identification in sugar beet using deep sequencing of DNA from phenotypic pools selected from breeding panels
por: Ries, David, et al.
Publicado: (2016)

Correction: U87MG Decoded: The Genomic Sequence of a Cytogenetically Aberrant Human Cancer Cell Line
por: Clark, Michael James, et al.
Publicado: (2018)

PoPoolation2: identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq)
por: Kofler, Robert, et al.
Publicado: (2011)

Fish mutant, where is thy phenotype?
por: Balciunas, Darius
Publicado: (2018)

Quality Control Test for Sequence-Phenotype Assignments
por: Ortiz, Maria Teresa Lara, et al.
Publicado: (2015)

A Direct Phenotypic Comparison of siRNA Pools and Multiple Individual Duplexes in a Functional Assay
por: Parsons, Brendon D., et al.
Publicado: (2009)

What’s old is new again: yeast mutant screens in the era of pooled segregant analysis by genome sequencing
por: Curtin, Chris, et al.
Publicado: (2016)

Comparative whole genome sequencing reveals phenotypic tRNA gene duplication in spontaneous Schizosaccharomyces pombe La mutants
por: Iben, James R., et al.
Publicado: (2011)

A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing
por: Frank, Ulrike, et al.
Publicado: (2019)

A distinctive oral phenotype points to FAM20A mutations not identified by Sanger sequencing
por: Poulter, James A., et al.
Publicado: (2015)

Exome Sequencing Identifies Genetic Variants Associated with Extreme Manifestations of the Cardiovascular Phenotype in Marfan Syndrome
por: Jimenez, Yanireth, et al.
Publicado: (2022)

Fabry disease with atypical phenotype identified by massively parallel sequencing in early-onset kidney failure
por: de Haan, Amber, et al.
Publicado: (2022)

Phenotype Driven Analysis of Whole Genome Sequencing Identifies Deep Intronic Variants that Cause Retinal Dystrophies by Aberrant Exonization
por: Di Scipio, Matteo, et al.
Publicado: (2020)

Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii
por: Dutcher, Susan K., et al.
Publicado: (2012)

A Broad Phenotypic Screen Identifies Novel Phenotypes Driven by a Single Mutant Allele in Huntington’s Disease CAG Knock-In Mice
por: Hölter, Sabine M., et al.
Publicado: (2013)

Molecular-Genetic Mapping of Zebrafish Mutants with Variable Phenotypic Penetrance
por: Jain, Roshan A., et al.
Publicado: (2011)

Genotyping by Sequencing of Cultivated Lentil (Lens culinaris Medik.) Highlights Population Structure in the Mediterranean Gene Pool Associated With Geographic Patterns and Phenotypic Variables
por: Pavan, Stefano, et al.
Publicado: (2019)

Structural variants in the barley gene pool: precision and sensitivity to detect them using short-read sequencing and their association with gene expression and phenotypic variation
por: Weisweiler, Marius, et al.
Publicado: (2022)

Chemical interference with DSIF complex formation lowers synthesis of mutant huntingtin gene products and curtails mutant phenotypes
por: Deng, Ning, et al.
Publicado: (2022)

Destabilizing Protein Polymorphisms in the Genetic Background Direct Phenotypic Expression of Mutant SOD1 Toxicity
por: Gidalevitz, Tali, et al.
Publicado: (2009)

Genome sequencing-based coverage analyses facilitate high-resolution detection of deletions linked to phenotypes of gamma-irradiated wheat mutants
por: Komura, Shoya, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_5a3h239ql48j90ddmvh9j24l02