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The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the eff...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042665/ https://www.ncbi.nlm.nih.gov/pubmed/21403830 http://dx.doi.org/10.1155/2010/380948 |
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author | Safrankova, Barbora Gajdova, Silvie Kubala, Lukas |
author_facet | Safrankova, Barbora Gajdova, Silvie Kubala, Lukas |
author_sort | Safrankova, Barbora |
collection | PubMed |
description | The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA. |
format | Text |
id | pubmed-3042665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30426652011-03-14 The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes Safrankova, Barbora Gajdova, Silvie Kubala, Lukas Mediators Inflamm Research Article The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA. Hindawi Publishing Corporation 2010 2011-02-09 /pmc/articles/PMC3042665/ /pubmed/21403830 http://dx.doi.org/10.1155/2010/380948 Text en Copyright © 2010 Barbora Safrankova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Safrankova, Barbora Gajdova, Silvie Kubala, Lukas The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title_full | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title_fullStr | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title_full_unstemmed | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title_short | The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes |
title_sort | potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042665/ https://www.ncbi.nlm.nih.gov/pubmed/21403830 http://dx.doi.org/10.1155/2010/380948 |
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