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The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes

The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the eff...

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Detalles Bibliográficos
Autores principales: Safrankova, Barbora, Gajdova, Silvie, Kubala, Lukas
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042665/
https://www.ncbi.nlm.nih.gov/pubmed/21403830
http://dx.doi.org/10.1155/2010/380948
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author Safrankova, Barbora
Gajdova, Silvie
Kubala, Lukas
author_facet Safrankova, Barbora
Gajdova, Silvie
Kubala, Lukas
author_sort Safrankova, Barbora
collection PubMed
description The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA.
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spelling pubmed-30426652011-03-14 The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes Safrankova, Barbora Gajdova, Silvie Kubala, Lukas Mediators Inflamm Research Article The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA. Hindawi Publishing Corporation 2010 2011-02-09 /pmc/articles/PMC3042665/ /pubmed/21403830 http://dx.doi.org/10.1155/2010/380948 Text en Copyright © 2010 Barbora Safrankova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Safrankova, Barbora
Gajdova, Silvie
Kubala, Lukas
The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title_full The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title_fullStr The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title_full_unstemmed The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title_short The Potency of Hyaluronan of Different Molecular Weights in the Stimulation of Blood Phagocytes
title_sort potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042665/
https://www.ncbi.nlm.nih.gov/pubmed/21403830
http://dx.doi.org/10.1155/2010/380948
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