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Transcriptome signature of the adult mouse choroid plexus

BACKGROUND: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as o...

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Autores principales: Marques, Fernanda, Sousa, João C, Coppola, Giovanni, Gao, Fuying, Puga, Renato, Brentani, Helena, Geschwind, Daniel H, Sousa, Nuno, Correia-Neves, Margarida, Palha, Joana A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042978/
https://www.ncbi.nlm.nih.gov/pubmed/21349147
http://dx.doi.org/10.1186/2045-8118-8-10
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author Marques, Fernanda
Sousa, João C
Coppola, Giovanni
Gao, Fuying
Puga, Renato
Brentani, Helena
Geschwind, Daniel H
Sousa, Nuno
Correia-Neves, Margarida
Palha, Joana A
author_facet Marques, Fernanda
Sousa, João C
Coppola, Giovanni
Gao, Fuying
Puga, Renato
Brentani, Helena
Geschwind, Daniel H
Sousa, Nuno
Correia-Neves, Margarida
Palha, Joana A
author_sort Marques, Fernanda
collection PubMed
description BACKGROUND: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as one of the barriers that separate the brain from the periphery and in the production of cerebrospinal fluid. METHODS: This work extends further what is known about the mouse CP transcriptome through a microarray analysis of CP tissue from normal mice under physiological conditions. RESULTS: We found that the genes most highly expressed are those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. On the other hand, genes encoding for immune mediators are among those with lower expression in basal conditions. In addition, we found genes known to be relevant during brain development, and not previously identified to be expressed in the CP, including those encoding for various axonal guidance and angiogenesis molecules and for growth factors. Some of these are known to influence the neural stem cell niche in the subventricular zone, highlighting the involvement of the CP as a likely modulator of neurogenesis. Interestingly, our observations confirm that the CP transcriptome is unique, displaying low homology with that of other tissues. Of note, we describe here that the closest similarity is with the transcriptome of the endothelial cells of the blood-brain barrier. CONCLUSIONS: Based on the data presented here, it will now be possible to further explore the function of particular proteins of the CP secretome in health and in disease.
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spelling pubmed-30429782011-02-23 Transcriptome signature of the adult mouse choroid plexus Marques, Fernanda Sousa, João C Coppola, Giovanni Gao, Fuying Puga, Renato Brentani, Helena Geschwind, Daniel H Sousa, Nuno Correia-Neves, Margarida Palha, Joana A Fluids Barriers CNS Research BACKGROUND: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as one of the barriers that separate the brain from the periphery and in the production of cerebrospinal fluid. METHODS: This work extends further what is known about the mouse CP transcriptome through a microarray analysis of CP tissue from normal mice under physiological conditions. RESULTS: We found that the genes most highly expressed are those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. On the other hand, genes encoding for immune mediators are among those with lower expression in basal conditions. In addition, we found genes known to be relevant during brain development, and not previously identified to be expressed in the CP, including those encoding for various axonal guidance and angiogenesis molecules and for growth factors. Some of these are known to influence the neural stem cell niche in the subventricular zone, highlighting the involvement of the CP as a likely modulator of neurogenesis. Interestingly, our observations confirm that the CP transcriptome is unique, displaying low homology with that of other tissues. Of note, we describe here that the closest similarity is with the transcriptome of the endothelial cells of the blood-brain barrier. CONCLUSIONS: Based on the data presented here, it will now be possible to further explore the function of particular proteins of the CP secretome in health and in disease. BioMed Central 2011-01-18 /pmc/articles/PMC3042978/ /pubmed/21349147 http://dx.doi.org/10.1186/2045-8118-8-10 Text en Copyright ©2011 Marques et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Marques, Fernanda
Sousa, João C
Coppola, Giovanni
Gao, Fuying
Puga, Renato
Brentani, Helena
Geschwind, Daniel H
Sousa, Nuno
Correia-Neves, Margarida
Palha, Joana A
Transcriptome signature of the adult mouse choroid plexus
title Transcriptome signature of the adult mouse choroid plexus
title_full Transcriptome signature of the adult mouse choroid plexus
title_fullStr Transcriptome signature of the adult mouse choroid plexus
title_full_unstemmed Transcriptome signature of the adult mouse choroid plexus
title_short Transcriptome signature of the adult mouse choroid plexus
title_sort transcriptome signature of the adult mouse choroid plexus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042978/
https://www.ncbi.nlm.nih.gov/pubmed/21349147
http://dx.doi.org/10.1186/2045-8118-8-10
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