Cargando…
Histomorphometric and sympathetic innervation of the human internal thoracic artery
INTRODUCTION: Internal thoracic artery (ITA) is an established arterial graft for the coronary artery by‐pass surgery. Special micro‐anatomical features of the ITA wall may protect it from age related pathological changes. One of the complications seen after coronary artery bypass grafting is vasosp...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044562/ https://www.ncbi.nlm.nih.gov/pubmed/21437449 http://dx.doi.org/10.1590/S1807-59322011000100023 |
_version_ | 1782198737115807744 |
---|---|
author | Reddy, Sreenivasulu Kumar, Pramod Prasad, Keerthana |
author_facet | Reddy, Sreenivasulu Kumar, Pramod Prasad, Keerthana |
author_sort | Reddy, Sreenivasulu |
collection | PubMed |
description | INTRODUCTION: Internal thoracic artery (ITA) is an established arterial graft for the coronary artery by‐pass surgery. Special micro‐anatomical features of the ITA wall may protect it from age related pathological changes. One of the complications seen after coronary artery bypass grafting is vasospasm. Sympathetic nerves may be involved in vasospasm. OBJECTIVE: To ascertain the sympathetic innervation of the internal thoracic artery and to assess the effect of aging on this artery by histomorphometry. METHOD: Fifty‐four human internal thoracic artery samples were collected from 27 cadavers (19 male and 8 female) with ages of 19 to 83 years. Samples were divided into three age groups: G1, 19–40 years; G2, 41–60 years; G3, ≥61 years. Sections (thickness 5 µm) of each sample were taken and stained with hematoxylin–eosin and Verhoeff–Van Gieson stains. Five of fifty‐four samples were processed for tyrosine hydroxylase immunostaining. RESULTS: The thickness of the tunica intima was found to be constant in all age groups, whereas the thickness of the tunica media decreased in proportion to age. Verhoeff–Van Gieson staining showed numerous elastic laminae in the tunica media. Significant differences (p<0.0001) in the number of elastic laminae were found between G1 with G2 cadavers, between G2 and G3 cadavers and between G3 and G1 cadavers. Tyrosine hydroxylase immunostaining demonstrated sympathetic fibers, located mainly in the tunica adventitia and the adventitia–media border. The sympathetic nerve fiber area and sympathetic index were found to be 0.0016 mm(2) and 0.012, respectively. DISCUSSION: Histology of the ITA showed features of the elastic artery. This may be associated with lower incidence of Atherosclerosis or intimal hyperplasia in ITA samples even in elderly cases. Low sympathetic index (0.012) of ITA may be associated with fewer incidences of sympathetic nervous systems problems (vasospasm) of the ITA. CONCLUSION: Sympathetic nerve fibers are present in the adventitia of the internal thoracic artery. This is an elastic artery, although anatomically it is considered to be medium‐sized. The sympathetic index may be used for analysis of sympathetic nerve fiber‐related problems of the internal thoracic artery. |
format | Text |
id | pubmed-3044562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-30445622011-02-24 Histomorphometric and sympathetic innervation of the human internal thoracic artery Reddy, Sreenivasulu Kumar, Pramod Prasad, Keerthana Clinics (Sao Paulo) Basic Research INTRODUCTION: Internal thoracic artery (ITA) is an established arterial graft for the coronary artery by‐pass surgery. Special micro‐anatomical features of the ITA wall may protect it from age related pathological changes. One of the complications seen after coronary artery bypass grafting is vasospasm. Sympathetic nerves may be involved in vasospasm. OBJECTIVE: To ascertain the sympathetic innervation of the internal thoracic artery and to assess the effect of aging on this artery by histomorphometry. METHOD: Fifty‐four human internal thoracic artery samples were collected from 27 cadavers (19 male and 8 female) with ages of 19 to 83 years. Samples were divided into three age groups: G1, 19–40 years; G2, 41–60 years; G3, ≥61 years. Sections (thickness 5 µm) of each sample were taken and stained with hematoxylin–eosin and Verhoeff–Van Gieson stains. Five of fifty‐four samples were processed for tyrosine hydroxylase immunostaining. RESULTS: The thickness of the tunica intima was found to be constant in all age groups, whereas the thickness of the tunica media decreased in proportion to age. Verhoeff–Van Gieson staining showed numerous elastic laminae in the tunica media. Significant differences (p<0.0001) in the number of elastic laminae were found between G1 with G2 cadavers, between G2 and G3 cadavers and between G3 and G1 cadavers. Tyrosine hydroxylase immunostaining demonstrated sympathetic fibers, located mainly in the tunica adventitia and the adventitia–media border. The sympathetic nerve fiber area and sympathetic index were found to be 0.0016 mm(2) and 0.012, respectively. DISCUSSION: Histology of the ITA showed features of the elastic artery. This may be associated with lower incidence of Atherosclerosis or intimal hyperplasia in ITA samples even in elderly cases. Low sympathetic index (0.012) of ITA may be associated with fewer incidences of sympathetic nervous systems problems (vasospasm) of the ITA. CONCLUSION: Sympathetic nerve fibers are present in the adventitia of the internal thoracic artery. This is an elastic artery, although anatomically it is considered to be medium‐sized. The sympathetic index may be used for analysis of sympathetic nerve fiber‐related problems of the internal thoracic artery. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011-01 /pmc/articles/PMC3044562/ /pubmed/21437449 http://dx.doi.org/10.1590/S1807-59322011000100023 Text en Copyright © 2011 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Basic Research Reddy, Sreenivasulu Kumar, Pramod Prasad, Keerthana Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title | Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title_full | Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title_fullStr | Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title_full_unstemmed | Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title_short | Histomorphometric and sympathetic innervation of the human internal thoracic artery |
title_sort | histomorphometric and sympathetic innervation of the human internal thoracic artery |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044562/ https://www.ncbi.nlm.nih.gov/pubmed/21437449 http://dx.doi.org/10.1590/S1807-59322011000100023 |
work_keys_str_mv | AT reddysreenivasulu histomorphometricandsympatheticinnervationofthehumaninternalthoracicartery AT kumarpramod histomorphometricandsympatheticinnervationofthehumaninternalthoracicartery AT prasadkeerthana histomorphometricandsympatheticinnervationofthehumaninternalthoracicartery |