Cargando…
Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system
The Beckman Coulter UniCel® DxH 800 is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC, white blood cell (WBC) differential, nucleated red blood cell (NRBC), and reticulocyte analysis. Evaluation of this instrument was perfor...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044820/ https://www.ncbi.nlm.nih.gov/pubmed/20491996 http://dx.doi.org/10.1111/j.1751-553X.2010.01239.x |
_version_ | 1782198781746348032 |
---|---|
author | HEDLEY, B D KEENEY, M CHIN-YEE, I BROWN, W |
author_facet | HEDLEY, B D KEENEY, M CHIN-YEE, I BROWN, W |
author_sort | HEDLEY, B D |
collection | PubMed |
description | The Beckman Coulter UniCel® DxH 800 is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC, white blood cell (WBC) differential, nucleated red blood cell (NRBC), and reticulocyte analysis. Evaluation of this instrument was performed in our 800-bed tertiary care hospital and specifically centered upon the correlation of WBC, NRBC, and platelet (PLT) enumeration when compared to a predicate analyzer, the Coulter® LH 780, and flow cytometry (FCM) reference methods. Of particular interest were those samples with morphologically confirmed interference and extreme leukocytosis (evaluated with respect to red blood cell parameter correction). The sample set (n = 272) consisted of morphologically normal and hematologically abnormal patients. Correlation of the WBC, PLT, and NRBC showed r(2) values of 0.994, 0.985, and 0.910 for the DxH 800 vs. FCM, respectively. The presence of interfering particles did not affect the accuracy of the DxH 800 with respect to WBC counts. The DxH 800 showed accurate PLT and NRBC counts in the clinically significant low range when compared to FCM. Compared to the LH 780, flagging rates were significantly reduced (NRBC flag), or equivalent (WBC, PLT flag) on the DxH 800. The DxH 800 demonstrated higher sensitivity and specificity for PLTs and NRBCs and achieved a lower NRBC false negative rate compared to the LH 780. The UniCel® DxH 800 represents a significant improvement to previous impedance analyzers in accurately detecting the presence of NRBCs at counts >1/100 WBC. Furthermore, it provides accurate PLT and WBC counts in the presence of interference and improved NRBC flagging efficiency when compared to the LH 780. Correction of red blood cell parameters is appropriate and accurate in cases of extreme leukocytosis. |
format | Text |
id | pubmed-3044820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-30448202011-03-02 Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system HEDLEY, B D KEENEY, M CHIN-YEE, I BROWN, W Int J Lab Hematol Original Articles The Beckman Coulter UniCel® DxH 800 is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC, white blood cell (WBC) differential, nucleated red blood cell (NRBC), and reticulocyte analysis. Evaluation of this instrument was performed in our 800-bed tertiary care hospital and specifically centered upon the correlation of WBC, NRBC, and platelet (PLT) enumeration when compared to a predicate analyzer, the Coulter® LH 780, and flow cytometry (FCM) reference methods. Of particular interest were those samples with morphologically confirmed interference and extreme leukocytosis (evaluated with respect to red blood cell parameter correction). The sample set (n = 272) consisted of morphologically normal and hematologically abnormal patients. Correlation of the WBC, PLT, and NRBC showed r(2) values of 0.994, 0.985, and 0.910 for the DxH 800 vs. FCM, respectively. The presence of interfering particles did not affect the accuracy of the DxH 800 with respect to WBC counts. The DxH 800 showed accurate PLT and NRBC counts in the clinically significant low range when compared to FCM. Compared to the LH 780, flagging rates were significantly reduced (NRBC flag), or equivalent (WBC, PLT flag) on the DxH 800. The DxH 800 demonstrated higher sensitivity and specificity for PLTs and NRBCs and achieved a lower NRBC false negative rate compared to the LH 780. The UniCel® DxH 800 represents a significant improvement to previous impedance analyzers in accurately detecting the presence of NRBCs at counts >1/100 WBC. Furthermore, it provides accurate PLT and WBC counts in the presence of interference and improved NRBC flagging efficiency when compared to the LH 780. Correction of red blood cell parameters is appropriate and accurate in cases of extreme leukocytosis. Blackwell Publishing Ltd 2011-02 /pmc/articles/PMC3044820/ /pubmed/20491996 http://dx.doi.org/10.1111/j.1751-553X.2010.01239.x Text en Copyright © 2011 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Original Articles HEDLEY, B D KEENEY, M CHIN-YEE, I BROWN, W Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title | Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title_full | Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title_fullStr | Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title_full_unstemmed | Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title_short | Initial performance evaluation of the UniCel® DxH 800 Coulter® cellular analysis system |
title_sort | initial performance evaluation of the unicel® dxh 800 coulter® cellular analysis system |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044820/ https://www.ncbi.nlm.nih.gov/pubmed/20491996 http://dx.doi.org/10.1111/j.1751-553X.2010.01239.x |
work_keys_str_mv | AT hedleybd initialperformanceevaluationoftheuniceldxh800coultercellularanalysissystem AT keeneym initialperformanceevaluationoftheuniceldxh800coultercellularanalysissystem AT chinyeei initialperformanceevaluationoftheuniceldxh800coultercellularanalysissystem AT brownw initialperformanceevaluationoftheuniceldxh800coultercellularanalysissystem |