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In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts

BACKGROUND: In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS) extracts by using various solvents (hexane, chloroform-ethanol (1/1), ethyl acetate, butanol and water). We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by mea...

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Autores principales: Kammoun, Maher, Miladi, Sonia, Ali, Yassine Ben, Damak, Mohamed, Gargouri, Youssef, Bezzine, Sofiane
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045348/
https://www.ncbi.nlm.nih.gov/pubmed/21310091
http://dx.doi.org/10.1186/1476-511X-10-30
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author Kammoun, Maher
Miladi, Sonia
Ali, Yassine Ben
Damak, Mohamed
Gargouri, Youssef
Bezzine, Sofiane
author_facet Kammoun, Maher
Miladi, Sonia
Ali, Yassine Ben
Damak, Mohamed
Gargouri, Youssef
Bezzine, Sofiane
author_sort Kammoun, Maher
collection PubMed
description BACKGROUND: In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS) extracts by using various solvents (hexane, chloroform-ethanol (1/1), ethyl acetate, butanol and water). We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA). RESULTS: The water extract exhibits the highest inhibitory effect with an IC(50 )= 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB) even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1) and ethyl acetate (IC(50 )= 0.274 and 0.326 mg/ml, respectively). Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g). This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively), mostly responsible for the antioxidant activity. CONCLUSION: A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.
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spelling pubmed-30453482011-03-01 In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts Kammoun, Maher Miladi, Sonia Ali, Yassine Ben Damak, Mohamed Gargouri, Youssef Bezzine, Sofiane Lipids Health Dis Research BACKGROUND: In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS) extracts by using various solvents (hexane, chloroform-ethanol (1/1), ethyl acetate, butanol and water). We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA). RESULTS: The water extract exhibits the highest inhibitory effect with an IC(50 )= 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB) even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1) and ethyl acetate (IC(50 )= 0.274 and 0.326 mg/ml, respectively). Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g). This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively), mostly responsible for the antioxidant activity. CONCLUSION: A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds. BioMed Central 2011-02-11 /pmc/articles/PMC3045348/ /pubmed/21310091 http://dx.doi.org/10.1186/1476-511X-10-30 Text en Copyright ©2011 Kammoun et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kammoun, Maher
Miladi, Sonia
Ali, Yassine Ben
Damak, Mohamed
Gargouri, Youssef
Bezzine, Sofiane
In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title_full In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title_fullStr In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title_full_unstemmed In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title_short In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts
title_sort in vitro study of the pla2 inhibition and antioxidant activities of aloe vera leaf skin extracts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045348/
https://www.ncbi.nlm.nih.gov/pubmed/21310091
http://dx.doi.org/10.1186/1476-511X-10-30
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