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Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish

BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related...

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Autores principales: Chun, Chang Zoon, Remadevi, Indu, Schupp, Marcus-Oliver, Samant, Ganesh Vinayak, Pramanik, Kallal, Wilkinson, George Albert, Ramchandran, Ramani
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045372/
https://www.ncbi.nlm.nih.gov/pubmed/21364913
http://dx.doi.org/10.1371/journal.pone.0014732
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author Chun, Chang Zoon
Remadevi, Indu
Schupp, Marcus-Oliver
Samant, Ganesh Vinayak
Pramanik, Kallal
Wilkinson, George Albert
Ramchandran, Ramani
author_facet Chun, Chang Zoon
Remadevi, Indu
Schupp, Marcus-Oliver
Samant, Ganesh Vinayak
Pramanik, Kallal
Wilkinson, George Albert
Ramchandran, Ramani
author_sort Chun, Chang Zoon
collection PubMed
description BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp (+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli (+) etsrp (+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp (+) cell numbers at the LPM. CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.
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spelling pubmed-30453722011-03-01 Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish Chun, Chang Zoon Remadevi, Indu Schupp, Marcus-Oliver Samant, Ganesh Vinayak Pramanik, Kallal Wilkinson, George Albert Ramchandran, Ramani PLoS One Research Article BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp (+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli (+) etsrp (+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp (+) cell numbers at the LPM. CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish. Public Library of Science 2011-02-25 /pmc/articles/PMC3045372/ /pubmed/21364913 http://dx.doi.org/10.1371/journal.pone.0014732 Text en Chun et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chun, Chang Zoon
Remadevi, Indu
Schupp, Marcus-Oliver
Samant, Ganesh Vinayak
Pramanik, Kallal
Wilkinson, George Albert
Ramchandran, Ramani
Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title_full Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title_fullStr Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title_full_unstemmed Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title_short Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
title_sort fli (+) etsrp (+) hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045372/
https://www.ncbi.nlm.nih.gov/pubmed/21364913
http://dx.doi.org/10.1371/journal.pone.0014732
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