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Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045372/ https://www.ncbi.nlm.nih.gov/pubmed/21364913 http://dx.doi.org/10.1371/journal.pone.0014732 |
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author | Chun, Chang Zoon Remadevi, Indu Schupp, Marcus-Oliver Samant, Ganesh Vinayak Pramanik, Kallal Wilkinson, George Albert Ramchandran, Ramani |
author_facet | Chun, Chang Zoon Remadevi, Indu Schupp, Marcus-Oliver Samant, Ganesh Vinayak Pramanik, Kallal Wilkinson, George Albert Ramchandran, Ramani |
author_sort | Chun, Chang Zoon |
collection | PubMed |
description | BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp (+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli (+) etsrp (+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp (+) cell numbers at the LPM. CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish. |
format | Text |
id | pubmed-3045372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30453722011-03-01 Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish Chun, Chang Zoon Remadevi, Indu Schupp, Marcus-Oliver Samant, Ganesh Vinayak Pramanik, Kallal Wilkinson, George Albert Ramchandran, Ramani PLoS One Research Article BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp (+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli (+) etsrp (+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp (+) cell numbers at the LPM. CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish. Public Library of Science 2011-02-25 /pmc/articles/PMC3045372/ /pubmed/21364913 http://dx.doi.org/10.1371/journal.pone.0014732 Text en Chun et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chun, Chang Zoon Remadevi, Indu Schupp, Marcus-Oliver Samant, Ganesh Vinayak Pramanik, Kallal Wilkinson, George Albert Ramchandran, Ramani Fli (+) etsrp (+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title |
Fli
(+)
etsrp
(+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title_full |
Fli
(+)
etsrp
(+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title_fullStr |
Fli
(+)
etsrp
(+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title_full_unstemmed |
Fli
(+)
etsrp
(+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title_short |
Fli
(+)
etsrp
(+) Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish |
title_sort | fli
(+)
etsrp
(+) hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045372/ https://www.ncbi.nlm.nih.gov/pubmed/21364913 http://dx.doi.org/10.1371/journal.pone.0014732 |
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